AB1784P

Anti-Dopamine D1A Receptor Antibody, 4th Extracellular Domain

Chemicon^®, from rabbit

• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity purified immunoglobulin
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: rat
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable; immunohistochemistry: suitable; western blot: suitable
• NCBI accession no.: NM_000794.3
• UniProt accession no.: P21728
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... DRD1(1812)

Descripción

Specificity

Dopamine D1A Receptor (D1R). The rat D1A receptor is a 446 amino acid, G-protein coupled transmembrane receptor protein (Monsma, 1990; Zhou, 1990; Zhou, 1992; O′Dowd, 1990). The immunogen sequence has 88% sequence similarity with human and 77% with porcine.

Immunogen

A nine amino acid peptide corresponding to the 4th extracellular domain of the rat D1A receptor. The immunogen is absolutely conserved in the mouse D1A receptor and shows 88% homology with human and 77% with porcine and bovine D1A receptors. The peptide shows no significant sequence similarity with other dopamine receptors (D1B, D2-D5).

Epitope: 4th Extracellular Domain

Application

Detect Dopamine D1A Receptor using this Anti-Dopamine D1A Receptor Antibody, 4th Extracellular Domain validated for use in ELISA, IH & WB.

Immunohistochemistry: 2-10 μg/mL on paraformaldehyde fixed tissue sections. D1R has been localized in various regions of the rat brain (Huang, 1992; O′Connel, 1995)

Western blot: 1-10 μg/mL (Chemiluminescence technique). An antibody made to this peptide has been shown to detect 2 proteins of 50 and 75 kDa in solubilized hippocampal membranes. The 75 kDa band was not visible in striatum (Huang, 1992; O′Connel, 1995). Approximately 100-400 μg of protein/lane should be loaded to visualize the 75 kDa band (Huang, 1992; O′Connel, 1995) that is present in relatively small amounts.

ELISA: 0.5-1.0 μg/mL using 1 μg/mL of control peptide per well.

The immunogen peptide is available (Cat. No. AG266) for use in ELISA or antibody blocking studies. AG266 is not recommended for use as a western blot positive control.

Optimal working dilutions must be determined by end user.

Research Category
Neuroscience

Research Sub Category
Neurotransmitters & Receptors

Physical form

Affinity purified immunoglobulin. Liquid in PBS with 0.2% BSA and 0.05% sodium azide.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 6 months from date of receipt. Avoid repeated freeze/thaw cycles.

Analysis Note

Control
POSITIVE CONTROL: rat striatal membranes (western blot)

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable