70527

100 mM IPTG Solution - Novagen

Convenient, ready-to-use solution

• Sinónimos: 100 mM IPTG Solution - Novagen, IPTG Solution, 100 mM, Isopropyl -β-D-1-thiogalactopyranoside, Isopropylthio -β-D-galactoside
• Número de CAS: 367-93-1
• MDL number: MFCD00063273
• UNSPSC Code: 12352200
• NACRES: NA.54

Propiedades

• Quality Level: 100
• form: solution
• mol wt: 238.30 g/mol
• manufacturer/tradename: Novagen^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• shipped in: wet ice
• storage temp.: −20°C
• SMILES string: S([C@@H]1O[C@@H]([C@@H]([C@@H]([C@H]1O)O)O)CO)C(C)C
• InChI: 1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1
• InChI key: BPHPUYQFMNQIOC-NXRLNHOXSA-N

Descripción

Application

100 mM IPTG Solution - Novagen has been used to:

• induce expression of an anti-CD11b-nanobody with a sortase recognition tag in Chalmers (WK6) cells[1]
• induce expression in calcium-independent sortase from BL21(DE3) cells[1]
• turn on bacterial protein production of the protein A/G-MNase[2]

Biochem/physiol Actions

Isopropyl-β-D-1-thiogalactopyranoside (IPTG) is an inducer of the lacoperon. It is highly recommended for blue/white screening using lacZα-complementation in appropriate vectors and host strains, for example, pSTBlue-1/NovaBlue. In addition, it is also used for inducing protein expression with the pET system and various lac promoter-derived expression systems. IPTG is supplied as a sterile aqueous solution and is free of dioxane. It comes in convenient volumes of 10 × 1.5 ml. IPTG is used in bacterial cultures to induce the expression of plasmid-based genes to produce recombinant proteins under the control of the lac promoter.[3]

Warning

Toxicity: Carcinogenic / Teratogenic (D)

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable