524751-M

Phosphodiesterase 7A1, Human, Recombinant, S. frugiperda

• Sinónimos: hPDE7A1
• Código UNSPSC: 12352203

Propiedades

• recombinante: expressed in baculovirus infected Sf21 cells
• Nivel de calidad: 100
• Formulario: liquid
• actividad específica: ≥0.5 U/μL
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze; avoid repeated freeze/thaw cycles
• temp. de almacenamiento: −70°C

Descripción

Descripción general

Full-length, recombinant, human phosphodiesterdase 7A1 (PDE7A1) expressed in S. frugiperda insect cells using a baculovirus expression system. PDE7A1 is required for T-cell activation induced by the costimulation of CD3 and CD28 receptors. Increased PDE7A1 correlates with a decrease in cAMP, increased IL-2, and increased cell proliferation. Supplied as a partially purified preparation of catalytically active enzyme useful for inhibitor and activator assays.

Advertencia

Toxicity: Standard Handling (A)

Definición de unidad

One unit is defined as the amount of enzyme that is required to convert 1 pmol cAMP to AMP per min at 30°C, pH 7.5.

Forma física

In 150 mM NaCl, 10 mM Tris, 0.1% NP-40, pH 8.3.

Reconstitución

Following initial thaw, aliquot and freeze (-70°C).

Otras notas

Han, P., et al. 2006. J. Biol. Chem.281, 15050.
Lugnier, C. 2006. Pharmacol Ther.109, 366.
Bloom, T.J., and Beavo, J.A. 1996. Proc. Natl. Acad. Sci. U S A93, 14188.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable