324740

Elastase Substrate V, Fluorogenic

≥95% (HPLC), powder, lyophilized solid, elastase substrate, Calbiochem^®

• Sinónimos: Elastase Substrate V, Fluorogenic, MeOSuc-AAPV-AMC
• Fórmula empírica (notación de Hill): C₃₁H₄₁N₅O₉
• Número de CAS: 72252-90-5
• Peso molecular: 627.69
• Número MDL: MFCD00077135
• Código UNSPSC: 12352200
• NACRES: NA.25

Propiedades

• Nombre del producto: Elastase Substrate V, Fluorogenic, Elastase Substrate V, Fluorogenic, CAS 72252-90-5, is a sensitive fluorogenic substrate for the quantitative determination of human leukocyte and porcine pancreatic elastase (K_m = 140 µM).
• Nivel de calidad: 200
• Ensayo: ≥95% (HPLC); ≥95% (TLC)
• Formulario: lyophilized solid; powder
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze; desiccated; protect from light
• color: white to off-white
• solubilidad: ethanol: 1 mg/mL; DMF: 5 mg/mL; DMSO: 5 mg/mL
• fluorescencia: λ_ex 360-380 nm; λ_em 430-460 nm
• Condiciones de envío: ambient
• temp. de almacenamiento: −20°C
• cadena SMILES: N1([C@@H](CCC1)C(=O)NC(=O)[C@@H](Nc2cc3c(cc2)C(=CC(=O)O3)C)C(C)C)C(=O)[C@@H](NC(=O)[C@@H](NC(=O)CCC(=O)OC)C)C
• InChI: 1S/C31H41N5O9/c1-16(2)27(34-20-9-10-21-17(3)14-26(39)45-23(21)15-20)30(42)35-29(41)22-8-7-13-36(22)31(43)19(5)33-28(40)18(4)32-24(37)11-12-25(38)44-6/h9-10,14-16,18-19,22,27,34H,7-8,11-13H2,1-6H3,(H,32,37)(H,33,40)(H,35,41,42)/t18-,19-,22-,27-/m0/s1
• Clave InChI: NHMQNKPYBPDCAY-NFHWZJRKSA-N

Descripción

Descripción general

Sensitive fluorogenic substrate for the quantitative determination of human leukocyte (K_m = 140 µM; K_cat/K_m = 120,000 s^-1 M^-1) and porcine pancreatic elastase. Not hydrolyzed by cathepsin G. Excitation max.: ~380 nm; emission max.: ~460 nm.

Sensitive fluorogenic substrate for the quantitative determination of human leukocyte and porcine pancreatic elastase (K_m = 140 µM; k_cat/K_m = 120,000 M^-1 s^-1). Not hydrolysed by cathepsin G.

Acciones bioquímicas o fisiológicas

Cell permeable: no

Km = 140 µM; kcat/Km = 120,000 M⁻¹ s⁻¹as substrates for human leukocyte and porcine pancreatic elastase

Primary Target
Sensitive fluorogenic substrate for the quantitative determination of human leukocyte and porcine pancreatic elastase

Product does not compete with ATP.

Reversible: no

Advertencia

Toxicity: Standard Handling (A)

Secuencia

MeOSuc-Ala-Ala-Pro-Val-AMC

Reconstitución

Following reconstitution, aliquot and freeze at -20°C. Stock solutions are stable for up to 2 months at -20°C.

Otras notas

Barrett, A.J. 1981. Methods Enzymol. 80, 581.
Castillo, M.J., et al. 1979. Anal. Biochem.99, 53.
Nakajima, K., et al. 1979. J. Biol. Chem.254, 4027.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable