324699

Elastase Substrate IV, Colorimetric

A specific substrate for pancreatic elastase.

• Sinónimos: Elastase Substrate IV, Colorimetric
• Fórmula empírica (notación de Hill): C₂₅H₃₄N₆O₉
• Peso molecular: 562.57
• NACRES: NA.54
• UNSPSC Code: 12352200
• Assay: ≥95% (HPLC)
• Form: lyophilized solid
• Storage condition: OK to freeze, avoid repeated freeze/thaw cycles, desiccated

Propiedades

• Quality Segment: 100
• assay: ≥95% (HPLC)
• form: lyophilized solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, avoid repeated freeze/thaw cycles, desiccated
• color: white to off-white
• solubility: ethanol: 1 mg/mL, water: soluble
• shipped in: ambient
• storage temp.: −20°C

Descripción

General description

A specific substrate for pancreatic elastase (K_m = 100 µM; K_cat/K_m = 35,000 s^-1 M^-1 for rat pancreatic elastase; K_m = 30 µM; K_cat/K_m = 351,000 s^-1 M^-1 for porcine pancreatic elastase). Cleavage of substrate can be monitored at ~405 nm.



Suggested protocol: see Largman, C. (1983)

Biochem/physiol Actions

Cell permeable: no

Primary Target
A specific substrate for pancreatic elastase

Product does not compete with ATP.

Reversible: no

km = 100 µM; kcat/ Km = 35,000 M-1 s-1 for rat pancreatic elastase; Km = 30 µM; kcat/Km = 35,000 M-1 s-1 for porcine

Physical form

45 mg D-mannitol and 5 mg substrate. Sold on the basis of substrate content.

Preparation Note

Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 3 months at 4°C.

Other Notes

Largman, L. 1983. Biochemistry22, 3763.
Del Mar, E.G., et al. 1980. Biochemistry19, 468.

Suc-Ala-Ala-Pro-Abu-pNA (Abu = L-α-Aminobutyric Acid)

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Información de seguridad

• Clase de almacenamiento: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable