251520-M

DAF-FM DA

A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM.

• Sinónimos: DAF-FM DA, 3-Amino, 4-aminomethyl-2ʹ,7ʹ-difluorofluorescein Diacetate
• Fórmula empírica (notación de Hill): C₂₅H₁₈F₂N₂O₇
• Número de CAS: 254109-22-3
• Peso molecular: 496.42
• Número MDL: MFCD16872050
• Código UNSPSC: 12352204
• NACRES: NA.25

Propiedades

• Nivel de calidad: 100
• Ensayo: ≥98% (HPLC)
• Formulario: solution
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze; protect from light
• color: pale yellow
• fluorescencia: λ_ex ~500 nm; λ_em ~515 nm
• temp. de almacenamiento: −20°C
• cadena SMILES: Fc1cc2c(cc1OC(=O)C)Oc3c(cc(c(c3)OC(=O)C)F)C42OC(=O)c5c4ccc(c5N)NC
• InChI: 1S/C25H18F2N2O7/c1-10(30)33-20-8-18-13(6-15(20)26)25(12-4-5-17(29-3)23(28)22(12)24(32)36-25)14-7-16(27)21(34-11(2)31)9-19(14)35-18/h4-9,29H,28H2,1-3H3
• Clave InChI: BEVHTVRRVVEMEF-UHFFFAOYSA-N

Descripción

Descripción general

A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM. Intracellularly releases DAF-FM (Cat. No. 251515) by the action of esterases. Reacts with NO, in the presence of O₂, resulting in a triazolo-fluorescein analog (DAF-FM T) that exhibits about 160-fold greater fluorescence quantum efficiency. The fluorescence is reported to be stable above pH 5.8.

Acciones bioquímicas o fisiológicas

Primary Target
Nitric oxide (NO) fluorescent indicator

Advertencia

Toxicity: Irritant (B)

Forma física

A 5 mM solution in DMSO.

Reconstitución

Following initial thaw, aliquot and freeze at (-20°C). Use immediately after dilution; discard any unused portion.

Otras notas

Kojima, H., et al. 2001. J. Neurochem.76, 1404.
Park. J.M., et al. 2001. Br. J. Pharmacol.132, 1876.
Itoh, Y., et al. 2000. Anal. Biochem.287, 203.
Kojima, H., et al. 1999. Angew Chem. Int. Ed.38, 3209.

Información legal

Manufactured by Daiichi Pure Chemicals Co., Ltd

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): 188.6 °F - closed cup - (Dimethylsulfoxide)
• Punto de inflamabilidad (°C): 87 °C - closed cup - (Dimethylsulfoxide)