1.50465

SeQuant^® ZIC^®-HILIC 200Å (5 µm) HPLC Columns

L × I.D. 150 mm x 0.075 mm capillay Column

• UNSPSC Code: 41115709
• NACRES: NB.21
• Particle size: 5 μm
• Matrix active group: ZIC-HILIC, ZIC-HILIC bonding phase
• Pore size: 200 Å pore size
• Matrix: fully porous particle

Propiedades

• Nombre del producto: SeQuant^® ZIC^®-HILIC^™ (5 µm, 200 Å) 150 x 0.075 mm, suitable for HPLC
• material: stainless steel column
• Quality Segment: 100
• agency: suitable for USP L114
• product line: SeQuant^®
• feature: endcapped: no
• analyte functional class(es): antibiotics, sweeteners, water soluble vitamins
• analyte chemical class(es): PFAS, alcohols / fermentation, aliphatic amines, alkaloids, amino acids, aromatic amines, carboxylic acids as methyl esters, esters, glycans, glycols, inorganic ions, monosaccharides, nitrosamines, nucleosides, oligonucleotides, organic phosphates, peptides, pesticides, phospholipids, polysaccharides, small carbohydrates, sugar alcohols, sulfonamides
• parameter: 400 bar pressure, 70 °C max. temp.
• technique(s): HPLC: suitable, LC/MS: suitable (Capillary LC), mass spectrometry (MS): suitable (High)
• column L × I.D.: 15 cm × 0.075 mm
• matrix: fully porous particle
• matrix active group: ZIC-HILIC, ZIC-HILIC bonding phase
• particle size: 5 μm
• pore size: 200 Å pore size
• operating pH: 2-7.5
• separation technique: hydrophilic interaction (HILIC)
• storage temp.: no temp limit

Descripción

General description

SeQuant^® ZIC^®-HILIC Columns are designed to retain and separate all types of polar and hydrophilic compounds and for robust chromatography with high selectivity and reproducibility. The silica-based ZIC^®-HILIC sorbent has a bonded stationary phase consisting of a highly polar, permanent zwitterion. Separation selectivity is favored by the 1:1 zwitterion charge balance, which makes the ZIC^®-HILIC Column overall neutral, with weak, but important, ionic interactions. Tuning of the selectivity on the ZIC^®-HILIC Column during method development is facilitated by the pH-inpendent permanent zwitterion, ensuring that only the analytes (and not the column) are affected during eluent optimization.The Pore size of 200 Å makes this column perfectly suitable for the separation of larger polar molecules under HILIC conditions.The column dimension of 250 mm length x 2.1 mm ID is the optimal choice for the separation of complex samples using a UHPLC or optimized HPLC Instrument providing low dwell volumes.Thanks to their favorable retention and robust performance, SeQuant^® HILIC columns have become extremely popular in numerous laboratories around the world. For example, US FDA (United States Food and Drug Administration) has recommended SeQuant^® ZIC^®-HILIC for analysis of melamine and related substances. SeQuant^® HILIC columns excel in stability and low bleed. These features make them particularly suitable for LC-MS applications where high background can lead to signal suppression and interference with quantitative measurements while also increasing instrument wear. Their high stability makes these high-performance HILIC columns also attractive for traditional HPLC applications.

Learn more about the Supelco HPLC Column portfolio and Method development:

Brochure: HPLC and UHPLC Column Selection Guide

Brochure: A Practical Guide to HPLC - Method Development Guide

Application

• 2-Homoectoine: An Additional Member of the Ectoine Family from Phyto- and Bacterioplankton Involved in Osmoadaptation.: This study explores the discovery of 2-homoectoine, a new member of the ectoine family, and its role in osmoadaptation in phyto- and bacterioplankton, highlighting its potential in biotechnological applications (Azizah and Pohnert, 2024).


• An efficient strategy with a synergistic effect of hydrophilic and electrostatic interactions for simultaneous enrichment of N- and O-glycopeptides.: The research presents a novel method for enriching glycopeptides using synergistic hydrophilic and electrostatic interactions, showcasing the efficiency of ZIC-HILIC columns in proteomics (Hu et al., 2024).


• Improved analysis ZIC-HILIC-HCD-Orbitrap method for mapping the glycopeptide by mass spectrometry.: This paper describes enhancements in glycopeptide mapping using a ZIC-HILIC-HCD-Orbitrap method, emphasizing its utility in detailed proteomic analyses (Li et al., 2023).


• Isomeric separation of native N-glycans using nano zwitterionic-hydrophilic interaction liquid chromatography column.: The study highlights the separation of isomeric N-glycans using nano-ZIC-HILIC columns, demonstrating advancements in glycomic profiling techniques (Daramola et al., 2023).

Legal Information

SeQuant is a registered trademark of Merck KGaA, Darmstadt, Germany

ZIC is a registered trademark of Merck KGaA, Darmstadt, Germany

ZIC-HILIC is a trademark of Merck KGaA, Darmstadt, Germany