14-383

Rho Assay Reagent (Rhotekin RBD, agarose), 650 µg

Specifically binds to & precipitates GTP-Rho, but not GDP-Rho from cell lysates. For use in Affinity Binding Assays.

• Sinónimos: Rho Assay Reagent, Rho Detection Reagent, Rhotekin RBD Assay Reagent
• UNSPSC Code: 12352200
• NACRES: NA.84
• eCl@ss: 32160405

Propiedades

• biological source: mouse
• Quality Segment: 100
• manufacturer/tradename: Upstate^®
• technique(s): affinity binding assay: suitable
• NCBI accession no.: NM_001015055.1, NM_001015056.1, NM_033046.2
• UniProt accession no.: Q9BST9
• shipped in: dry ice
• Gene Information: mouse ... RTKN(20166)

Descripción

General description

GST-tagged fusion protein, corresponding to residues 7-89 of mouse Rhotekin Rho Binding Domain, expressed in E.coli and bound to glutathione-agarose. Specifically binds to and precipitates GTP-Rho, but not GDP-Rho from cell lysates.

Product Source: Expressed in E. coli

Physical form

650 µg of recombinant protein in 1 mL glutathione-agarose slurry containing 45mM TRIS, pH 7.5, 0.45% Triton X-100, 135mM NaCl, 4.5mM MgCl2, 0.9mM dithiothreitol, 0.09mM PMSF, 0.9 µg/mL aprotinin, 0.9 µg/mL leupeptin, and 10% glycerol.

Preparation Note

Stable for 6 months at -70°C from date of receipt.

Analysis Note

Routinely evaluated by precipitating GTP-Rho from 3T3/A31 lysates, subsequent detection by immunoblot analysis using Anti-RhoA, clone 55 (05-778).

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Códigos de peligro: H412,EUH430
• Indicaciones de precaución: P201 - P202 - P273 - P391 - P405 - P501
• Clase de almacenamiento: 10 - Combustible liquids
• Palabra de advertencia: Danger
• Hazard Classifications: Aquatic Chronic 3 - ED ENV 1
• ¿Qué está pasando?: WGK 2