14-338
ROKα/ROCK-II Protein, active, rat, 10 µg
Active, rat, N-terminal, His-tagged, fusion protein corresponding to amino acids 2-543 of rat ROKa/ROCK-II. For use in Kinase Assays.
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
About This Item
Código UNSPSC:
12352202
eCl@ss:
32160405
NACRES:
NA.32
Productos recomendados
origen biológico
rat
Nivel de calidad
mol peso
Mw 66 kDa
fabricante / nombre comercial
Upstate®
técnicas
activity assay: suitable (kinase)
Nº de acceso NCBI
Nº de acceso UniProt
Condiciones de envío
dry ice
Información sobre el gen
rat ... ROCK1(81762)
Descripción general
N-terminal, His-tagged, fusion protein corresponding to amino acids 2-543 of rat ROKa/ROCK-II.
Note: The preparation contains numerous Sf21 cellular proteins which bind weakly to Ni-NTA agarose.
Note: The preparation contains numerous Sf21 cellular proteins which bind weakly to Ni-NTA agarose.
Product Source: Rat ROKa/ROCK-II expressed in Sf21 insect cells.
Acciones bioquímicas o fisiológicas
Protein Target: ROKα/ROCK-II
Target Sub-Family: AGC
Calidad
Rroutinely evaluated using the S6 Kinase Assay Kit
Forma física
10μg of enzyme in 2.3μl of 50mM Tris/HCl pH7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 2mM benzamidine, 0.4mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Ni-NTA agarose
Almacenamiento y estabilidad
6 months at -70°C
Otras notas
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Información legal
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Palabra de señalización
Warning
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Skin Sens. 1
Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 2
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Alexander Khromov et al.
The Journal of biological chemistry, 284(32), 21569-21579 (2009-06-18)
The reversible regulation of myosin light chain phosphatase (MLCP) in response to agonist stimulation and cAMP/cGMP signals plays an important role in the regulation of smooth muscle (SM) tone. Here, we investigated the mechanism underlying the inhibition of MLCP induced
Vincent Sauzeau et al.
Methods in molecular biology (Clifton, N.J.), 1527, 213-218 (2017-01-25)
Increased arterial tone and the resulting rise in peripheral vascular resistance are major determinants of the elevated arterial pressure in hypertension. The RhoA/Rho kinase signaling pathways are now recognized as a major regulator of vascular smooth muscle contraction and arterial
M Eto et al.
The Journal of biological chemistry, 276(31), 29072-29078 (2001-06-09)
Histamine stimulus triggers inhibition of myosin phosphatase-enhanced phosphorylation of myosin and contraction of vascular smooth muscle. In response to histamine stimulation of intact femoral artery, a smooth muscle-specific protein called CPI-17 (for protein kinase C-potentiated inhibitory protein for heterotrimeric myosin
David A Carlson et al.
Cell chemical biology, 25(10), 1195-1207 (2018-07-24)
Sustained vascular smooth muscle hypercontractility promotes hypertension and cardiovascular disease. The etiology of hypercontractility is not completely understood. New therapeutic targets remain vitally important for drug discovery. Here we report that Pim kinases, in combination with DAPK3, regulate contractility and
Cassandra L Hubert et al.
Journal of biomolecular screening, 8(4), 399-409 (2003-10-22)
The Rho-associated coiled-coil-containing protein serine/threonine kinases ROCK-I and ROCK-II are thought to play a major role in cytoskeletal dynamics by serving as downstream effectors of the Rho/Rac family of cytokine- and growth factor-activated small GTPases. As such, the ROCK family
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico