Suspend 25 g LB-Broth in 1 litre of DI water and autoclave for 15 min at 121 °C. pH: 7.0 ± 0.2 at 25 °C. The prepared broth is clear and yellowish-brown.
1.10285
LB Broth
GranuCult® plus, granular, pack of 500 g or 5 kg, for Escherichia coli
Sinónimos:
E. coli culture medium, GranuCult® plus LB broth (MILLER), Luria Bertani Broth, Luria Bertani Medium
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NACRES:
NA.74
UNSPSC Code:
41106213
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Servicio técnico
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Permítanos ayudarlesterility
non-sterile
Quality Segment
form
granular
manufacturer/tradename
GranuCult® plus
packaging
pack of 500 g or 5 kg
storage condition
dry media (Keep tightly closed)
technique(s)
microbiological culture: suitable
color
light yellow to brown
pH
6.8-7.2 (25 °C in H2O, after autoclaving)
application(s)
microbiology
pharmaceutical
storage temp.
15-25°C
suitability
Escherichia coli
General description
GranuCult® plus LB broth (MILLER) formulation supports the growth of Escherichia coli. Casein peptone and yeast extracts supply essential growth factors, such as nitrogen, carbon, sulfur, minerals, and vitamins.
Application
GranuCult® plus LB broth (MILLER) is suitable for the cultivation of E. coli in fermentation and molecular genetic studies.
Features and Benefits
- GranuCult® offers superior granulated culture media
- Safe and sustainable due to reduced risks associated with fine dust and toxic substance inhalation, resulting in a safer work environment
- Excellent wettability, solubility, and free-flowing properties
- Convenient, with minimal component separation and clumping, even under warm or humid conditions
- High batch-to-batch reproducibility
- Prolonged shelf life of up to five years
- High number of test strains exceeding all regulatory demands
- Granulation technology allows many supplements to be included, with no need to add these separately
Analysis Note
Appearance (clearness): clear
Appearance (color): yellowish to yellowish brown
pH-value (25 °C): 6.8 - 7.2
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)): ≤ 100
Inoculum on reference medium (Escherichia coli ATCC 11775 (WDCM 00090)): ≤ 100
Inoculum on reference medium (Escherichia coli DSM 6897 (DH5alpha)): ≤ 100
Inoculum on reference medium (Escherichia coli ATCC 47092 (BL21)): ≤ 100
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): good to very good
Growth (Escherichia coli ATCC 11775 (WDCM 00090)): good to very good
Growth (Escherichia coli DSM 6897 (DH5alpha)): good to very good
Growth (Escherichia coli ATCC 47092 (BL21)): good to very good
Incubation: 24 ± 2 hours at 37 ± 1 °C, aerobic
Appearance (color): yellowish to yellowish brown
pH-value (25 °C): 6.8 - 7.2
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)): ≤ 100
Inoculum on reference medium (Escherichia coli ATCC 11775 (WDCM 00090)): ≤ 100
Inoculum on reference medium (Escherichia coli DSM 6897 (DH5alpha)): ≤ 100
Inoculum on reference medium (Escherichia coli ATCC 47092 (BL21)): ≤ 100
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): good to very good
Growth (Escherichia coli ATCC 11775 (WDCM 00090)): good to very good
Growth (Escherichia coli DSM 6897 (DH5alpha)): good to very good
Growth (Escherichia coli ATCC 47092 (BL21)): good to very good
Incubation: 24 ± 2 hours at 37 ± 1 °C, aerobic
Other Notes
Replaces: 1.10285.5007
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
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Este artículo | |||
|---|---|---|---|
| description GranuCult® plus, granular, pack of 500 g or 5 kg, for Escherichia coli | description GranuCult® plus, granular, for Escherichia coli, pkg of 500 g | description Microbiologically tested | description Novagen®, granular, pack of 500 g or 5 kg, for Escherichia coli |
| suitability Escherichia coli | suitability Escherichia coli | suitability - | suitability Escherichia coli |
| technique(s) microbiological culture: suitable | technique(s) microbiological culture: suitable | technique(s) - | technique(s) microbiological culture: suitable |
| form granular | form granular | form - | form granular |
| sterility non-sterile | sterility non-sterile | sterility - | sterility - |
| application(s) microbiology | application(s) microbiology | application(s) food and beverages | application(s) microbiology |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 100 |
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