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07-145

Sigma-Aldrich

Anti-phospho-Histone H3 (Ser28) Antibody

from rabbit, purified by affinity chromatography

Sinónimos:

Histone H3.1t, H3/t, H3t, H3/g

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

origen biológico

rabbit

Nivel de calidad

forma del anticuerpo

affinity isolated antibody

tipo de anticuerpo

primary antibodies

clon

polyclonal

purificado por

affinity chromatography

reactividad de especies

human, mouse

técnicas

ChIP: suitable
dot blot: suitable
immunocytochemistry: suitable
inhibition assay: suitable (peptide)
western blot: suitable

Nº de acceso NCBI

Nº de acceso UniProt

Condiciones de envío

wet ice

modificación del objetivo postraduccional

phosphorylation (pSer28)

Información sobre el gen

human ... H3C1(8350)
mouse ... H3C1(360198)

Descripción general

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Especificidad

This antibody is expected to demonstrate broad species reactivity due to sequence conservation.
This antibody recognizes Histone H3 phosphorylated at Ser28.

Inmunógeno

Epitope: Histone H3 phosphorylated at Ser28
KLH-conjugated linear peptide corresponding to Histone H3 phosphorylated at Ser28.

Aplicación

Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones
Use Anti-phospho-Histone H3 (Ser28) Antibody (Rabbit Polyclonal Antibody) validated in WB, ICC, DB, PIA, ChIP to detect phospho-Histone H3 (Ser28) also known as H3F3, H3F3B, H3S28P.
Western Blot Analysis: A 1:2,000 dilution from a representative lot detected phospho-Histone H3 (Ser28) in 10 µg of untreated and colcemid treated HeLa acid extract.

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected phospho-Histone H3 (Ser28) in HeLa cells.

Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated phospho-Histone H3 (Ser28) in colcecmid treated HeLa cell lysate.

Dot Blot Specificity Analysis: A representative lot of this antibody detected peptides corresponding to phospho-Histone H3 (Ser28), but not peptides corresponding to other unmodified and modifed Histones.

Peptide Inhibition Analysis: A 1:500 dilution form a representative was blocked by phospho-Histone H3 (Ser28) peptides, but not non-specific peptides.

Calidad

Evaluated by Western Blot in HeLa acid extract.

Western Blot Analysis: A 1:2,000 dilution of this antibody detected phospho-Histone H3 (Ser28) in 10 µg of HeLa acid extract.

Descripción de destino

~17 kDa observed

Forma física

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Nota de análisis

Control
HeLa acid extract

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 1


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