06-1013

Anti-NET5 Antibody

from rabbit, purified by affinity chromatography

• Sinónimos: transmembrane protein 201, Spindle-associated membrane protein 1
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: ICC, WB
• Citations: 5

Propiedades

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: rat, human, mouse
• species reactivity (predicted by homology): orangutan
• technique(s): immunocytochemistry: suitable, western blot: suitable
• NCBI accession no.: NP_001010866.1
• UniProt accession no.: Q5SNT2
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... TMEM201(199953)

Descripción

General description

Transmembrane protein 201, also known as NET5, is a transmembrane protein of the nuclear envelope that is homologous to spindle-associated membrane protein 1 (SAMP1) in humans. NET5 is thought to be expressed on the inner nuclear membrane during interphase and move to the polar regions of the mitotic spindle during mitosis.

~72 and/or 43 kDa observed depending on lysate used. Isoform1: 72 kDa and Isoform 2: 43 kDa Uncharacterized band at ~28 kDa may be observed in some lysates.

Immunogen

KLH-conjugated linear peptide corresponding to human NET5.

Application

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Use Anti-NET5 Antibody (Rabbit Polyclonal Antibody) validated in WB, ICC to detect NET5 also known as transmembrane protein 201.

Western Blot Analysis: 1 µg/mL from a representative lot detected NET5 on 10 µg of rat heart tissue lysate.

Immunocytochemistry Analysis: A representative lot was used by an independent laboratory that detected NET5 in U2OS cells expressing various NET-GFP fusions. (Wilkie, G., et al. (2011) Mol. Cell Proteomics).

Physical form

Affinity purified

Purified rabbit polyclonal containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Human heart tissue lysate

Evaluated by Western Blot in human heart tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected NET5 on 10 µg of human heart tissue lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Clase de almacenamiento: 12 - Non Combustible Liquids
• ¿Qué está pasando?: WGK 1
• Punto de inflamación (°F): Not applicable
• Punto de inflamación (°C): Not applicable