05-583
Anti-CFTR Antibody, clone M3A7
clone M3A7, Upstate®, from mouse
Sinónimos:
ATP-binding cassette sub-family C, member 7, ATP-binding cassette transporter sub-family C member 7, cAMP-dependent chloride channel, cystic fibrosis transmembrane conductance regulator, cystic fibrosis transmembrane conductance regulator (ATP-binding ca
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Productos recomendados
origen biológico
mouse
Nivel de calidad
forma del anticuerpo
purified immunoglobulin
tipo de anticuerpo
primary antibodies
clon
M3A7, monoclonal
reactividad de especies
human
fabricante / nombre comercial
Upstate®
técnicas
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotipo
IgG1
Nº de acceso NCBI
Nº de acceso UniProt
Condiciones de envío
dry ice
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... CFTR(1080)
Descripción general
CFTR (Cystic Fibrosis Transmembrane Regulator) is an integral membrane protein shown to be involved in the transport of chloride ions. It is found on the surface of the epithelial cells lining the lungs and other organs. CFTR defects are the cause of cystic fibrosis. Cystic Fibrosis (CF) is a common lethal genetic disease caused by mutations of the gene coding for the cystic fibrosis transmembrane conductance factor, a cAMP regulated chloride channel. Approximately 70% of all CF cases share the deletion of a phenylalanine at position 508 (delta F508), which results in abnormal chloride transport. Since the CF mutation is lethal, most often by lung and liver disease, it raises the question of why this genetic disease remains as common as it is.
Especificidad
Other species not tested.
This antibody recognizes CFTR, Mr 170 kDa.
Inmunógeno
Epitope: a.a. 1365-1395
GST-fusion protein corresponding to residues 1197-1480 of human cystic fibrosis transmembrane conductance regulator (CFTR). The epitope maps within amino acids 1365-1395. Clone M3A7.
Aplicación
Detect CFTR using this Anti-CFTR Antibody, clone M3A7 validated for use in IH, IP & WB.
Immunoprecipitation:
This antibody has been reported to immunoprecipitate CFTR. (Kartner, N., 1998.)
Immunohistochemistry:
This antibody has been reported to immunostain CFTR in human pancreatic tissue sections. (Kartner, N., 1998.)
This antibody has been reported to immunoprecipitate CFTR. (Kartner, N., 1998.)
Immunohistochemistry:
This antibody has been reported to immunostain CFTR in human pancreatic tissue sections. (Kartner, N., 1998.)
Research Category
Neuroscience
Neuroscience
Research Sub Category
Ion Channels & Transporters
Ion Channels & Transporters
Calidad
Routinely evaluated by western blot on human T84 colon carcinoma epithelial RIPA cell lysates or from CFTR-transfected BHK2.
Western Blot Analysis:
0.5-2 µg/mL of this lot detected CFTR from 20-50 µg of human T84 colon carcinoma epithelial RIPA cell lysates. 0.5-2 µg/mL of a previous lot detected CFTR from CFTR-transfected BHK (Haardt, M., 1999).
Note: Do not boil the lysate. Instead incubate at 37°C for 30 minutes. CFTR can run as a diffuse protein on SDS-PAGE.
Western Blot Analysis:
0.5-2 µg/mL of this lot detected CFTR from 20-50 µg of human T84 colon carcinoma epithelial RIPA cell lysates. 0.5-2 µg/mL of a previous lot detected CFTR from CFTR-transfected BHK (Haardt, M., 1999).
Note: Do not boil the lysate. Instead incubate at 37°C for 30 minutes. CFTR can run as a diffuse protein on SDS-PAGE.
Descripción de destino
170 kDa
Forma física
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1 in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide and 30% glycerol.
Almacenamiento y estabilidad
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
Nota de análisis
Control
T84 cell lysate.
T84 cell lysate.
Otras notas
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Información legal
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Opcional
Referencia del producto
Descripción
Precios
Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 1
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Miquéias Lopes-Pacheco et al.
Chembiochem : a European journal of chemical biology, 17(6), 493-505 (2016-02-13)
We evaluated whether small molecule correctors could rescue four nucleotide-binding domain 1 (NBD1) mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene (A455E, S492F, ΔI507, and R560T). We first transfected Cos-7 cells (green monkey kidney cells) with A455E, S492F, ΔI507
Nicoletta Pedemonte et al.
American journal of physiology. Cell physiology, 298(4), C866-C874 (2010-01-08)
Cystic fibrosis (CF) is caused by mutations in the CFTR chloride channel. Deletion of phenylalanine 508 (F508del), the most frequent CF mutation, impairs the maturation and gating of the CFTR protein. Such defects may be corrected in vitro by pharmacological
CFTR mediates apoptotic volume decrease and cell death by controlling glutathione efflux and ROS production in cultured mice proximal tubules.
Sebastien l'Hoste,Abderrahmen Chargui,Radia Belfodil,Elisabeth Corcelle et al.
American Journal of Physiology: Renal Physiology null
M Haardt et al.
The Journal of biological chemistry, 274(31), 21873-21877 (1999-07-27)
Defective cAMP-stimulated chloride conductance of the plasma membrane of epithelial cell is the hallmark of cystic fibrosis (CF) and results from mutations in the cystic fibrosis transmembrane conductance regulator, CFTR. In the majority of CF patients, mutations in the CFTR
Mechanosensitive Cl- secretion in biliary epithelium mediated through TMEM16A.
Dutta, AK; Woo, K; Khimji, AK; Kresge, C; Feranchak, AP
American Journal of Physiology: Gastrointestinal and Liver Physiology null
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