04-858
Anti-Histone H4 Antibody, pan, rabbit monoclonal
culture supernatant, clone 62-141-13, Upstate®
Sinónimos:
H4, Histone H4
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Productos recomendados
origen biológico
rabbit
Nivel de calidad
forma del anticuerpo
culture supernatant
tipo de anticuerpo
primary antibodies
clon
62-141-13, monoclonal
reactividad de especies
Saccharomyces cerevisiae, human, vertebrates, chicken
fabricante / nombre comercial
Upstate®
técnicas
ChIP: suitable
multiplexing: suitable
western blot: suitable
isotipo
IgG
Nº de acceso NCBI
Nº de acceso UniProt
Condiciones de envío
dry ice
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... H4C1(8359)
Descripción general
This antibody recognizes histone H4 independant of any post-translational modifications.
Especificidad
Recognizes Histone H4.
Inmunógeno
Epitope: a.a. 25-28
Synthetic peptide corresponding to amino acids 17-28 of Histone H4. Epitope has been mapped to residues 25-28
Aplicación
Anti-Histone H4 Antibody, pan is a Rabbit Monoclonal Antibody for detection of Histone H4 also known as Histone H4 & has been validated in ChIP, WB, Mplex.
Immunoblot Analysis:
A 1:100,000 dilution of a previous lot detected unmodified recombinant Histone H4 protein (Catalog # 14-412) and Histone H4 in acid extracts from both untreated HeLa cells and cells treated with sodium butyrate (hyper-acetylated) or colcemid (hyper-phosphorylated) (Figure B).
Chromatin Immunoprecipitation:
An independent laboratory used a previous lot of this antibody in chromatin immunoprecipitation (Issac, C., 2006).
Multiplexing:
Serial dilutions of a previous lot were incubated with histone peptides containing various unmodified peptides spanning the aminoterminus of Histone H4 conjugated to Luminex® microspheres. The antibody recognized both modified and unmodified peptides.
A 1:100,000 dilution of a previous lot detected unmodified recombinant Histone H4 protein (Catalog # 14-412) and Histone H4 in acid extracts from both untreated HeLa cells and cells treated with sodium butyrate (hyper-acetylated) or colcemid (hyper-phosphorylated) (Figure B).
Chromatin Immunoprecipitation:
An independent laboratory used a previous lot of this antibody in chromatin immunoprecipitation (Issac, C., 2006).
Multiplexing:
Serial dilutions of a previous lot were incubated with histone peptides containing various unmodified peptides spanning the aminoterminus of Histone H4 conjugated to Luminex® microspheres. The antibody recognized both modified and unmodified peptides.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Calidad
Routinely evaluated by immunoblot using HeLa cell acid extract.
Descripción de destino
10 kDa
Ligadura / enlace
Replaces: 05-858
Forma física
Cultured supernantant in 0.05% sodium azide.
Almacenamiento y estabilidad
Stable for 1 year at -20°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
For maximum recovery of product, centrifuge the vial prior to removing the cap. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Nota de análisis
Control
Recombinant Histone H4 (Catalog # 14-412) or HeLa cell acid extract.
Recombinant Histone H4 (Catalog # 14-412) or HeLa cell acid extract.
Información legal
Luminex is a registered trademark of Luminex Corp
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Opcional
Referencia del producto
Descripción
Precios
Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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