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F2456

Sigma-Aldrich

Fluorescein (free acid)

Dye content 95%, Powder

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About This Item

Fórmula empírica (notación de Hill):
C20H12O5
Número de CAS:
2321-07-5
Peso molecular:
332.31
Número de índice de color:
45350:1
Beilstein:
94324
Número CE:
219-031-8
Número MDL:
MFCD00005050
Código UNSPSC:
12171500
ID de la sustancia en PubChem:
24894822
NACRES:
NA.47

Nombre del producto

Fluorescein (free acid), Dye content 95 %

Formulario

powder

composición

Dye content, 95%

técnicas

titration: suitable

color

orange to red-brown

mp

320 °C (lit.)

λmáx.

496 nm

ε (coeficiente de extinción)

≥12000 at 282-286 nm
≥39000 at 236-240 nm
≥70000 at 488-492 nm

aplicaciones

diagnostic assay manufacturing
hematology
histology

temp. de almacenamiento

room temp

cadena SMILES

Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5ccccc45)c1

InChI

1S/C20H12O5/c21-11-5-7-15-17(9-11)24-18-10-12(22)6-8-16(18)20(15)14-4-2-1-3-13(14)19(23)25-20/h1-10,21-22H

Clave InChI

GNBHRKFJIUUOQI-UHFFFAOYSA-N

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Descripción general

Fluorescein (free acid, CI 45350) is a xanthene dye. Fluorescein (free acid) molecule can be divided into two parts. One part contains xanthene moiety which acts as fluorophore and the other part of the molecule bears benzene moiety which is the fluorescence-controlling moiety.

Aplicación

Fluorescein (free acid) has been used as a staining agent in retinal imaging.
Fluorescein (free acid) is widely used as a fluorescent stain and trace for many living cells and tissue applications. It is also suitable as a marker for the study of motility of bile canaliculi. Some of the clinical applications are evaluating the ocular surface and delineation of brain regions lacking a blood-brain barrier to permit accurate surgical resection of malignant glioma. Fluorescein angiography helps investigate changes in retinal blood supply. Fluorescein also plays a role as a physiological tracer to characterize the salmon′s intestinal permeability (Schep et al., 1998), and for the fluorophotometric measurement of aqueous humor flow. It is widely employed as a quantum yield standard.

Pictogramas

Exclamation mark
GHS07

Palabra de señalización

Warning

Frases de peligro

H319

Consejos de prudencia

P305 + P351 + P338

Clasificaciones de peligro

Eye Irrit. 2

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
0000277631
0000277631
SHBP5589
MKCJ1475
10102PE

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J J Riesz et al.
Physical review. E, Statistical, nonlinear, and soft matter physics, 76(2 Pt 1), 021915-021915 (2007-10-13)
We report the transition dipole strength of eumelanin (the principal human photoprotective pigment) in the ultraviolet and visible. We have used both theoretical (density functional) and experimental methods to show that eumelanin is not an unusually strong absorber amongst organic
Lesley-Ann Gray et al.
The Journal of infectious diseases, 216(11), 1460-1470 (2017-10-14)
Rheumatic heart disease (RHD) after group A streptococcus (GAS) infections is heritable and prevalent in Indigenous populations. Molecular mimicry between human and GAS proteins triggers proinflammatory cardiac valve-reactive T cells. Genome-wide genetic analysis was undertaken in 1263 Aboriginal Australians (398
Mi Ju Son et al.
Medicine, 98(29), e16527-e16527 (2019-07-25)
Several studies have found that obesity is associated with atopic dermatitis (AD); however, the mechanisms underlying the association are largely unknown. This study aims to assess the association of AD with obesity in the Korean population and verify its mechanism
Karen A Fisher et al.
Chemistry (Weinheim an der Bergstrasse, Germany), 9(23), 5873-5878 (2003-12-16)
The uptake of the three species of the drug model fluorescein (fluorescein sodium salt (FNa), fluorescein free acid (F), and fluorescein diacetate (FDA)) by zeolite NaX and the mesoporous zeotype MCM-41 was investigated as well as their release rates into
Tasuku Ueno et al.
Journal of the American Chemical Society, 126(43), 14079-14085 (2004-10-28)
Rational design strategies based on practical fluorescence modulation mechanisms would enable us to rapidly develop novel fluorescence probes for target molecules. Here, we present a practical and general principle for modulating the fluorescence properties of fluorescein. We hypothesized that (a)
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