24308

β-DEX^™ 325

L × I.D. 30 m × 0.25 mm, d_f 0.25 μm

• Synonym(s): β-DEX^™ 325, 30M .25MM .25UM
• UNSPSC Code: 41115710
• eCl@ss: 32119290

Properties

• material: fused silica
• description: GC capillary column
• parameter: 30-230 °C temperature
• Beta value: 250
• df: 0.25 μm
• technique(s): gas chromatography (GC): suitable
• L × I.D.: 30 m × 0.25 mm
• matrix active group: non-bonded; 25% 2,3-di-O-methyl-6-O-TBDMS-β-cyclodextrin in SPB-20 poly(20% phenyl/80% dimethylsiloxane) phase
• application(s): agriculture; chemicals and industrial polymers; cleaning products; clinical; cosmetics; environmental; flavors and fragrances; food and beverages; forensics and toxicology; life science and biopharma; personal care; pharmaceutical (small molecule)
• column type: capillary chiral
• separation technique: chiral

Description

General description

The chiral stationary phase in β-DEX 325 columns contains 2,3-di-O-methyl-6-0-TBDMS-β-cyclodextrin embedded in an intermediate polarity phase. The Supelco β-DEX 325 is similar in both chemistry and use to the CHIRALDEX B-DM phase, the main difference being the concentration of the dimethyl-derivatized cyclodextrin that is doped into the polysiloxane carrier.

Application

ß-DEX^™-325 column was used in gas-liquid chromatography (GLC), for determination of (R)-1-O-acetyl-2-ethyl-1,3-propanediol, formed from 2-Ethyl-1,3-propanediol 1 and its related di-O-acetate, by partial chemoenzymatic acetylation and deacetylation, by Pseudomonas fluorescens lipase. ß -Dex^™-325 capillary column was also used for resolving 8-acetoxylinalool enantiomers, in an experimental study done in order to study the origin of the enantioselectivity in the biosynthesis of the lilac compounds in Actinidia arguta flowers.

Chem/Phys Resistance

Temp. Limits:

• 30 °C to 230 °C

Other Notes

We offer a variety of chromatography accessories including analytical syringes

Legal Information

DEX is a trademark of Sigma-Aldrich Co. LLC