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product name
Granzyme B Substrate,
Assay
≥95% (HPLC)
form
lyophilized
composition
Peptide Content, ≥85%
storage condition
protect from light
storage temp.
−20°C
Amino Acid Sequence
Ac-Ile-Glu-Pro-Asp-AMC
Application
Granzyme B Substrate (Ac-IEPD-AMC) is a fluorogenic substrate for the detection and assay of caspase 8 and granzyme B which is involved in the rapid induction of target cell apoptosis by CTL in cell-mediated immune response.
Biochem/physiol Actions
Granzymes belongs to serine proteinase family enzymes and it localizes along with perforin in granules. It is predominant in cytotoxic T cells. Granzyme B induces caspases dependent and independent cell death. It possesses specific cleavage action at aspartate residue in the substrate.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Veterinary immunology and immunopathology, 141(1-2), 168-172 (2011-03-26)
Granzyme B plays an important role in granule-mediated apoptosis by CTL. It is a well characterized component of the cytolytic machinery in mammals and a candidate for the evaluation of cytotoxic activity of CTL as an alternative to conventional cytotoxicity
Infection and immunity, 72(5), 2590-2597 (2004-04-23)
Human peripheral blood monocytes become apoptotic following phagocytosis and killing of Staphylococcus aureus. Although this type of monocyte apoptosis is known to be initiated by Fas-Fas ligand (FasL) interactions, the downstream signaling pathway has not been determined. In this work
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.), 225(2), 143-150 (2000-10-24)
Cell death induction by cytotoxic T lymphocytes (CTLs) is an important thesis for the understanding of tumor immunotherapy. In the current study we investigated the molecular machinery of CTL-induced cell death in human hepatocellular carcinoma cell lines (HCC lines). CTLs
Fundamental Immunology, 1083-1083 (2008)
Basic Concepts of Molecular Pathology, 32-32 (2009)
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