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SAB4200186

Sigma-Aldrich

Anti-DCLK1 antibody produced in rabbit

enhanced validation

~1.5 mg/mL, affinity isolated antibody

Synonym(s):

Anti-DCAMKL1, Anti-DCDC3A, Anti-DCLK, Anti-doublecortin and CaM kinase-like 1

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.44

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

~81 kDa

species reactivity

rat, mouse, human

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

concentration

~1.5 mg/mL

technique(s)

western blot: 1.5-3.0 μg/mL using extracts of postnatal mouse brain (S1 fraction), postnatal rat brain (S1 fraction) and of HEK-293T cells over-expressing human DCLK1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... DCLK1(9201)
mouse ... Dclk1(13175)
rat ... Dclk1(83825)

General description

Anti-DCLK1 is produced in rabbit using as immunogen a synthetic peptide corresponding to human DCLK1, conjugated to KLH. DCLK1 (doublecortin like kinase 1) is also known as DCLK, DCAMKL1, DCDC3A and CLICK1. DCLK1 consists of an N-terminal doublecortin (DC) domain, responsible for its localization to microtubules and a C-terminal Ser/Thr kinase domain.
DCLK1 is a neuronal transmembrane enzyme that may regulate cortical development, nerve cell migration and microtubule polymerization. This enzyme is similar to DCX, but DCLK1 additionally functions as a Ca2+/calmodulin-dependent protein kinase that can phosphorylate myelin basic protein and itself . Anti-DCLK1 antibody is specific for human, rat and mouse DCLK1. In immunoblotting, detection of the DCLK1 band is specifically inhibited by the DCLK1 immunizing peptide.

Immunogen

The corresponding sequence is highly conserved (single amino acid substitution) in rat and mouse DCLK1.

Application

Anti-DCLK1 antibody is suitable for use in western blot (1.5-3.0 μg/mL using extracts of postnatal mouse brain (S1 fraction), postnatal rat brain (S1 fraction) and HEK-293T cells over-expressing human DCLK1). The antibody may also be used for immunoblot (approx. 81 kDa) assays.

Biochem/physiol Actions

Doublecortin-like kinase1 (DCLK1) is widely expressed in post-mitotic neurons in the developing nervous system and important for neuronal migration to cerebral cortex. DCLK1 is highly expressed during embryogenesis and is localized to neuronal growth cones where it associates with the microtubule cytoskeleton and regulates microtubule polymerization. DCLK1 kinase domain is susceptible to cleavage by the Ca2+ -dependent protease calpain, suggesting that a Ca2+ -responsive mechanism is involved in regulating the DCLK1 kinase activity during embryogenesis. DCLK1 has been identified as a putative intestinal stem cell marker.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Expression, characterization, and gene knockdown of zebrafish doublecortin-like protein kinase
Shimomura S, et al.
Archives of Biochemistry and Biophysics, 463(2), 218-230 (2007)
Prabhu Ramamoorthy et al.
Biomedicines, 9(5) (2021-05-01)
Triple negative breast cancer (TNBC) is observed in ~15% of breast cancers and results in poor survival and increased distant metastases. Within the tumor are present a small portion of cancer stem cells that drive tumorigenesis and metastasis. In this
Alternative transcripts of Dclk1 and Dclk2 and their expression in doublecortin knockout mice
Tuy F P D, wt al.
Developmental Neuroscience, 30(1-3), 171-186 (2008)
Cleavage of Doublecortin-like Kinase by Calpain Releases an Active Kinase Fragment from a Microtubule Anchorage Domain
Burgess H A and Reiner O
The Journal of biological chemistry, 276(39), 36397-36403 (2001)
DCAMKL1 encodes a protein kinase with homology to doublecortin that regulates microtubule polymerization
Lin P T, et al.
The Journal of Neuroscience, 20(24), 9152-9161 (2000)

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