P8045
Protein G-Biotin from Streptococcus sp.
recombinant, expressed in unspecified host, lyophilized powder
Synonym(s):
Biotinylated Protein G, Protein G Biotin, Protein G-Biotin
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About This Item
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recombinant
expressed in unspecified host
Quality Level
conjugate
biotin conjugate
form
lyophilized powder
composition
Protein, ~90% biuret
extent of labeling
2-4 mol d-biotin per mol protein
technique(s)
direct ELISA: 1:70,000 using human IgG
storage temp.
2-8°C
General description
Protein G binds to antibodies reversibly and thus, it is considered in antibody purification. It is a 20 kDa protein.[1]
Protein G is an immunoglobulin (IgG) specific bacterial cell wall protein isolated from Streptococci (group G). Native proteins G consist of two IgG-binding domains and albumin and cell surface binding sites.[2][3] Biotin is a water soluble vitamin that acts as a coenzyme for carboxylase enzyme reactions. Biotin deficiency results in deregulation of metabolic pathways and various physiological impairments. Biotin deficiency is known to cause birth anomalies indicating its important role in development and differentiation of embryos.[4][5]
Specificity
Binds IgG only from most mammalian species except cat; binds chicken IgG.
Application
Protein G-Biotin from Streptococcus sp. has been used during incubation for synergistic immunocapture of antigen which was performed using streptavidin-coated magnetic beads.[6]
Protein G-Biotin from Streptococcus sp. has been used in electrochemical biosensing, for the attachment of antibodies with different specificities.[7]
Packaging
Package size based on protein content
Other Notes
Truncated - lacks albumin, Fab and membrane binding sites.
Preparation Note
Prepared using biotinamidocaproate N-hydroxysuccinimide which incorporates an aminocaproyl spacer.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Ayumi Taniguchi et al.
Journal of nutritional science and vitaminology, 53(6), 457-463 (2008-01-19)
It is well known that biotin deficiency causes morphological anomalies in hatchlings of fowl. An abundance of biotin in the yolk, therefore, is greatly required for maintaining reproductive functions. Although there is growing evidence for the molecular significance of the
Principles of Bacterial Detection: Biosensors, Recognition Receptors and Microsystems: Biosensors, Recognition Receptors, and Microsystem, 21(7), 360-360 (2008)
Electrochemical biosensing based on universal affinity biocomposite platforms
Zacco E, et al.
Biosensors And Bioelectronics, 21(7), 1291-1301 (2006)
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The effects of orientating pairs of synergistic monoclonal antibodies (mAb) on binding of human chorionic gonadotropin (hCG) was studied by radioimmunoassay (RIA), enzyme-linked immunosorbent assay (ELISA) and surface plasmon resonance (SPR). Antibody synergy towards hCG required two functionally intact antibodies
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