P6407

Poly-ᴅ-Lysine Hydrobromide

synthetic, mol wt 70,000-150,000, powder, γ-irradiated, suitable for cell culture, BioXtra

• Synonym(s): PDL
• Linear Formula: D-Lys-(D-Lys)_n-D-Lys · xHBr
• CAS Number: 27964-99-4
• MDL number: MFCD00131934
• UNSPSC Code: 12352202
• PubChem Substance ID: 24898764
• NACRES: NA.75

Properties

• product name: Poly-D-lysine hydrobromide, mol wt 70,000-150,000, lyophilized powder, γ-irradiated, BioReagent, suitable for cell culture
• biological source: synthetic (organic)
• Quality Level: 200
• sterility: γ-irradiated
• product line: BioReagent; BioXtra
• form: lyophilized powder
• mol wt: 70,000-150,000
• packaging: pkg of 10 X 5 mg; pkg of 5 mg
• concentration: 0.016—0.032 mmol lysine
• technique(s): cell culture | mammalian: suitable
• surface coverage: 4 μg/cm²
• impurities: <10% water (by Karl Fischer)
• solubility: H₂O: soluble 50 mg/mL, clear, colorless
• shipped in: ambient
• storage temp.: −20°C
• SMILES string: O=C(C)[C@@](NC)([H])CCCCN.[Br]
• InChI: 1S/C6H14N2O2.BrH/c7-4-2-1-3-5(8)6(9)10;/h5H,1-4,7-8H2,(H,9,10);1H
• InChI key: MEXAGTSTSPYCEP-UHFFFAOYSA-N

Description

Application

Poly-D-lysine hydrobromide has been used:

• as a coating for plates used in culturing rat glial precursor cells (RGPCS).
• in coating flasks and plates in culturing tanycytes
• in preparing the surface of coverslips for facilitating cell attachment

Poly-D-lysine polymers can be used in preparing surfaces for cell attachment. The D-lysine polymers can also be used with cells that digest poly-L-lysine polymers and cause an excessive uptake of L-lysine.

This product is recommended as a cell culture substratum when using 0.5 - 1.0 mL of a 0.1 mg/mL solution to coat 25 cm². Lower molecular weight versions of the product are less viscous, but high more molecular weight versions provide more attachment sites per molecule.

Biochem/physiol Actions

Poly-D-lysine (PDL) hydrobromide is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. After absorption to the culture surface, poly-D-lysine increases the number of positively charged cell binding sites.

Features and Benefits

• Sterilized using gamma (γ) radiation.
• Sterile Poly-D-Lysine solutions can be stored for three years when stored at 2-8˚C.

Components

Poly-D-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-D-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the ß structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.

Caution

Sterile solutions are stable for up to 2 years when stored at 2-8°C. It should be stored desiccated at -20°C.

Preparation Note

This product has a molecular weight of 70,000-150,000 and is cell culture tested and gamma-irradiated. To remove the HBr, dissolve this product in a neutral buffer and dialyze to remove the salts. In general, to use this product as an attachment factor, add 50 mL of sterile tissue culture grade water to 5 mg of poly-lysine, and aseptically coat the surface with 1 mL per 25 cm² of solution. After 5 minutes, remove the solution through aspiration and thoroughly rinse the surface. Let dry for two hours before introducing cells and medium.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves