P4334
Anti-Protein Kinase Cα antibody produced in rabbit
whole antiserum
Synonym(s):
Anti-PKCα
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About This Item
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biological source
rabbit
conjugate
unconjugated
antibody form
whole antiserum
antibody product type
primary antibodies
clone
polyclonal
mol wt
antigen 80 kDa
contains
15 mM sodium azide
species reactivity
mouse, rat
technique(s)
microarray: suitable
western blot: 1:20,000 using mouse NIH 3T3 fibroblast lysate
western blot: 1:50,000 using rat brain extract
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
mouse ... Prkca(18750)
rat ... Prkca(24680)
General description
Anti-Protein Kinase Cα is produced in rabbit using a synthetic peptide conjugated to keyhole limpet hemocyanin (KLH) as the immunogen. PKC is a phospholipid dependent enzyme, activated by the lipid 1,2-diacylglycerol (DAG), an intracellular second messenger produced from hydrolysis of inositol phospholipids, in response to a variety of hormones, growth factors, and neurotransmitters.
PKC α is a conventional isoform of Protein Kinase C (76-93 kD) and a member of serine/threonine (Ser/Thr) protein kinases. It is expressed ubiquitously in tissues and is the major isoform of PKC in fibroblasts. Stimulation and overexpression of PKC α increases the growth rate of cells in culture. Anti-Protein Kinase C α antibody is useful for studying expression of PKC α in normal and neoplastic tissues. It is also useful for detecting PKC α isoenzyme in brain tissue and cell extracts. Furthermore, it can be used in chemiluminescence detection systems to detect PKC α. Rabbit anti-protein kinase c α antibody reacts specifically with PKC α (80 kD) of rat brain extract or NIH3T3 mouse fibroblast lysate.
Specificity
The antibody reacts in immunoblotting with PKCα in rat brain extract. Staining is specifically inhibited with PKCα immunizing peptide (659-672).
Immunogen
Synthetic peptide corresponding to amino acids from the C-terminal variable (V5) region of rat PKC α conjugated to KLH.
Application
Anti-Protein Kinase C α has been used in immunoblotting and immunohistochemistry.
Anti-Protein Kinase Cα antibody produced in rabbit has been used in light microscopic—immunocytochemical analysis and immunofluorescent labelling.
Biochem/physiol Actions
Protein Kinase C (PKC) plays a crucial role in signal transduction leading to cellular regulation, cell growth and differentiation, oncogenesis and modulation of neurotransmission. PKC is a phospholipid dependent enzyme, activated by the lipid 1,2-diacylglycerol (DAG), an intracellular second messenger produced as a result from hydrolysis of inositol phospholipids, in response to a variety of hormones, growth factors and neurotransmitters. PKC is also the major cellular receptor for the tumor promoting phorbol esters. PKC action is mediated by binding to specific receptors for activated C-kinase (RACKs) and through the phosphorylation of several cellular substrates. Overexpression and stimulation of PKC α leads to enhanced growth rate of cells in culture. Anti-Protein Kinase C α antibody is useful for studying expression of PKC α in normal and neoplastic tissues.
Physical form
Supplied as a liquid containing 15 mM sodium azide as preservative
Storage and Stability
For continuous use, store at 2-8 °C. For extended storage freeze in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
13 - Non Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Koen Jansen et al.
Neuropeptides, 37(1), 57-65 (2003-03-15)
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Glosmann M et al
Journal of Vision, 8, 1-12 (2008)
K Almholt et al.
The Biochemical journal, 337 ( Pt 2), 211-218 (1999-01-12)
The alpha isoform of protein kinase C (PKCalpha) is a ubiquitous protein kinase, which, upon activation, translocates rapidly from the cytoplasm to the plasma membrane. To follow this translocation, PKCalpha was tagged with a highly fluorescent derivative of green fluorescent
Protein kinase C: structural and spatial regulation by phosphorylation, cofactors, and macromolecular interactions
Newton AC
Chemical Reviews, 101(8), 2353-2364 (2001)
Mu Qiao et al.
Frontiers in molecular neuroscience, 8, 81-81 (2016-01-19)
Understanding how the nervous system functions requires mapping synaptic connections between neurons. Several methods are available for imaging neurons connected by chemical synapses, but few enable marking neurons connected by electrical synapses. Here, we demonstrate that a peptide transporter, Pept2
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