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P2401

Sigma-Aldrich

Pectinase from Rhizopus sp.

greener alternative

powder, 400-800 units/g solid

Synonym(s):

Macerozyme R-10, Poly-(1,4-α-D-galacturonide) glycanohydrolase, Polygalacturonase

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About This Item

CAS Number:
9032-75-1
Enzyme Commission number:
3.2.1.15 (BRENDA, IUBMB)
EC Number:
232-885-6
MDL number:
MFCD00131809
UNSPSC Code:
12352204
NACRES:
NA.54

form

powder

Quality Level

200

specific activity

400-800 units/g solid

greener alternative product characteristics

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

greener alternative category

, Enabling

storage temp.

−20°C

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General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in cellulosic ethanol research. For more information see the article in biofiles and Enzymes for Alternative Energy Research.

Pectinase, also known as pectinolytic enzyme, is predominantly found in bacteria, fungi and plants. Polygalacturonase is one of the highly abundant enzymes among all the pectinolytic enzymes. Pectinase has a wide range of industrial applications including, fruit juice extraction and its clarification. In addition, it is also used in vegetable oil extraction, tea and coffee fermentations, bleaching of paper, in poultry feed additives and in alcoholic beverages and food industries.

Application

Pectinase from Rhizopus sp has been used for enzymatic digestion of leaves.
Used in plant protoplast preparation to digest cell wall prior to organelle isolation.

Biochem/physiol Actions

Pectinases hydrolyses pectin, which is a component of the cell wall.They may attack methyl-esterified pectin or de-esterified pectin. It is a source of pectinase activity, also containing cellulase and hemicellulase activities.

Unit Definition

One unit will liberate 1.0 μmole of galacturonic acid from polygalacturonic acid per min at pH 4.0 at 25 °C.

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Microbial pectinolytic enzymes: a review
Jayani Ranveer Singh, et al.
Process. Biochem., 40(9), 2931-2944 (2005)
Isolation of intact sub-dermal secretory cavities from Eucalyptus
Goodger JQ, et al.
Plant methods, 6(1), 20-20 (2010)
Pectinase production by Neurospora crassa: purification and biochemical characterization of extracellular polygalacturonase activity
Maria de Lourdes T. M. Polizeli, et al.
Microbiology, 137, 1815-1823 (1991)
Sheetal K Karnik et al.
Plant physiology, 138(4), 1967-1981 (2005-07-26)
Homologs of peroxin 16 genes (PEX16) have been identified only in Yarrowia lipolytica, humans (Homo sapiens), and Arabidopsis (Arabidopsis thaliana). The Arabidopsis gene (AtPEX16), previously reported as the SSE1 gene, codes for a predicted 42-kD membrane peroxin protein (AtPex16p). Lin
Enzyme disintegration with spatial resolution reveals different distributions of sludge extracellular polymer substances
Lu Fan, et al.
Biotechnology for Biofuels, 9(1), 29-29 (2016)
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Protocols

Enzymatic Assay of Pectinase

To measure pectinase activity, a titrimetric stop reaction assay is used. One unit of pectinase will liberate 1 μmol of galacturonic acid from poly-galacturonic acid per hour at pH 4.0 at 25 °C.

Enzymatic Assay of Pectinase

To measure pectinase activity, a titrimetric stop reaction assay is used. One unit of pectinase will liberate 1 μmol of galacturonic acid from poly-galacturonic acid per hour at pH 4.0 at 25 °C.

Enzymatic Assay of Pectinase

To measure pectinase activity, a titrimetric stop reaction assay is used. One unit of pectinase will liberate 1 μmol of galacturonic acid from poly-galacturonic acid per hour at pH 4.0 at 25 °C.

Enzymatic Assay of Pectinase

To measure pectinase activity, a titrimetric stop reaction assay is used. One unit of pectinase will liberate 1 μmol of galacturonic acid from poly-galacturonic acid per hour at pH 4.0 at 25 °C.

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