L2145

L-Leucine-7-amido-4-methylcoumarin hydrochloride

fluorogenic, ≥98% (TLC), powder

• Synonym(s): H-L-Leu-AMC HCl
• Empirical Formula (Hill Notation): C₁₆H₂₀N₂O₃ · HCl
• CAS Number: 62480-44-8
• Molecular Weight: 324.80
• UNSPSC Code: 12352204
• PubChem Substance ID: 24896303
• NACRES: NA.32
• MDL number: MFCD00043262

Properties

• Product Name: L-Leucine-7-amido-4-methylcoumarin hydrochloride,
• assay: ≥98% (TLC)
• Quality Segment: 200
• form: powder
• solubility: methanol: 50 mg/mL, clear, colorless to faintly yellow
• fluorescence: λ_ex 327 nm; λ_em 349 nm (pH 8.0), λ_ex 380 nm; λ_em 440 nm (Reaction product)
• storage temp.: −20°C
• SMILES string: Cl.CC(C)C[C@H](N)C(=O)Nc1ccc2C(C)=CC(=O)Oc2c1
• InChI: 1S/C16H20N2O3.ClH/c1-9(2)6-13(17)16(20)18-11-4-5-12-10(3)7-15(19)21-14(12)8-11;/h4-5,7-9,13H,6,17H2,1-3H3,(H,18,20);1H/t13-;/m0./s1
• InChI key: VCRXITKKWBOQRZ-ZOWNYOTGSA-N

Description

General description

L-Leucine-7-amido-4-methylcoumarin hydrochloride (Leu-AMC) is a fluorogenic peptidyl substrate for leucine aminopeptidase, an extracellular enzyme found abundantly in natural aquatic systems. This enzyme is one of the various enzymes used by bacteria for protein hydrolysis.

Application

L-Leucine-7-amido-4-methylcoumarin hydrochloride has been used:

• as a substrate in fluorescence-based soil assays
• to determine leucine aminopeptidase activity of both Plasmodium falciparum M1 (PfA-M1) and PfA-M17 enzymes

L-Leucine-7-amido-4-methylcoumarin hydrochloride (Leu-AMC) as a substrate analog to determine leucine aminopeptidase activity

Safety Information

• Storage Class: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• PPE (personal protective equipment): Eyeshields, Gloves, type N95 (US)