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K0253

Sigma-Aldrich

Keratin from human epidermis

aqueous solution

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
12352202
NACRES:
NA.61

biological source

human epidermis

Assay

≥90% (SDS-PAGE)

form

urea solution

technique(s)

ELISA: suitable

UniProt accession no.

storage temp.

2-8°C

Gene Information

human ... KRT1(3848)

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General description

Keratins are characteristic intermediate filament proteins of the epithelia. In human, keratin is classified into type I and type II. About 54 genes encodes for the keratin protein. The keratin 1 (KRT1) gene is mapped to human chromosome 12q13.13. It is the primary component of hair, skin and nails.

Application

Keratin from human epidermis has been used:
  • as an analyte and a cross-reactant in the antibody-immobilized chip for testing selectivity coefficients
  • as a standard in extracellular matrix (ECM) protein in in vitro protein degradation assays.
  • asa a standard in enzyme linked immunosorbent assay (ELISA)

Biochem/physiol Actions

Keratins are important for the mechanical stability and integrity of epithelial cells and tissues. Some keratins also have regulatory functions and are involved in intracellular signaling pathways, e.g. protection from stress, wound healing, and apoptosis. This diverse group of cytoskeletal scaffolding proteins form intermediate filament networks which provide structural support to keratinocytes that maintain the integrity of the skin. Expression of keratin genes is usually regulated by differentiation of the epidermal cells within the stratifying squamous epithelium.

Physical form

Solution in 8 M urea, 50 mM Tris, 0.1 M β-mercaptoethanol and 0.1% sodium azide, pH 8.4

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Skin Sens. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Christian Mozet et al.
Anticancer research, 33(6), 2415-2424 (2013-06-12)
The hedgehog signaling pathway (HH) is involved in tumorigenesis in a variety of human malignancies. In head and neck squamous cell carcinomas (HNSCC), Hh overexpression was associated with poor prognosis. Therefore, we analyzed the effect of Hh signaling blockade with
A Pena et al.
Optics express, 13(17), 6667-6667 (2005-08-22)
We present corrected versions of the list of authors and of Section 2.1.
Christopher J Day et al.
PLoS neglected tropical diseases, 15(2), e0009136-e0009136 (2021-02-26)
Mycobacterium ulcerans is the causative agent of the chronic, necrotizing skin disease Buruli ulcer. Modes of transmission and molecular mechanisms involved in the establishment of M. ulcerans infections are poorly understood. Interactions with host glycans are often crucial in bacterial
Comparison of biomarkers in exhaled breath condensate and bronchoalveolar lavage
Jackson AS, et al.
American Journal of Respiratory and Critical Care Medicine, 175(3), 222-227 (2007)
Keratin-based Biomaterials and Bioproducts, 5-5 (2017)

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