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HPA036788

Sigma-Aldrich

Anti-HAT1 antibody produced in rabbit

enhanced validation

Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution

Synonym(s):

Anti-Histone acetyltransferase 1, Anti-KAT1

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About This Item

UNSPSC Code:
12352203
Human Protein Atlas Number:
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

product line

Prestige Antibodies® Powered by Atlas Antibodies

form

buffered aqueous glycerol solution

species reactivity

mouse, human, rat

enhanced validation

orthogonal RNAseq
RNAi knockdown
Learn more about Antibody Enhanced Validation

technique(s)

immunoblotting: 0.04-0.4 μg/mL
immunofluorescence: 0.25-2 μg/mL
immunohistochemistry: 1:200-1:500

immunogen sequence

KFKINKQHARRVYEILRLLVTDMSDAEQYRSYRLDIKRRLISPYKKKQRDLAKMRKCLRPEELTNQMNQIEISMQHEQLEESFQELVEDYRRVIERLA

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... HAT1(8520)

General description

The gene HAT1 (histone acetyltransferase 1) is mapped to human chromosome 2q31.1. It is a key member of HATs. It is an evolutionarily conserved protein and a type B histone acetyltransferase.

Immunogen

histone acetyltransferase 1 recombinant protein epitope signature tag (PrEST)

Application

All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry.

The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies® protocols and other useful information.

Biochem/physiol Actions

HAT1 (histone acetyltransferase 1) is responsible for histone acetylation, H4K5 (histone 4 lysine 5) and H4K12. MicroRNA miR-486 targets HAT1, thereby inhibiting the expression of ABCA1 (ATP-binding cassette transporter A1). These events suppress cholesterol efflux in THP-1 (human monocytic cell line) macrophages. In esophageal carcinoma, HAT1 is responsible for cell growth. Absence of HAT1 causes G2/M cell cycle arrest, leading to growth inhibition. HAT1 also controls DNA repair by homologous recombination.

Features and Benefits

Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:
  • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
  • Protein array of 364 human recombinant protein fragments.

Linkage

Corresponding Antigen APREST79052

Physical form

Solution in phosphate buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide.

Legal Information

Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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MiR-486 regulates cholesterol efflux by targeting HAT1.
Liu D, et al.
Biochemical and Biophysical Research Communications, 472, 418-424 (2016)
Novel candidate genes and regions for childhood apraxia of speech identified by array comparative genomic hybridization.
Laffin JJ, et al.
Genetics in Medicine : Official Journal of the American College of Medical Genetics, 14, 928-936 (2012)
RNAi screening identifies HAT1 as a potential drug target in esophageal squamous cell carcinoma.
Xue L, et al.
International Journal of Clinical and Experimental Pathology, 7, 3898-3907 (2014)
Histone acetyltransferase 1 promotes homologous recombination in DNA repair by facilitating histone turnover.
Yang X, et al.
The Journal of Biological Chemistry, 288, 18271-18282 (2013)

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