H7039

3-Hydroxy-3-methylglutaryl-CoA reductase human

solution

• Synonym(s): HMGCoA Reductase, HMGR
• Enzyme Commission number: 1.1.1.34 (BRENDA, IUBMB)
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• Assay: ≥90% (SDS-PAGE)
• Quality Level: 200
• form: solution
• specific activity: 2-8 units/mg protein
• mol wt: ~76 kDa (for the fusion protein)
• UniProt accession no.: P04035
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... HMGCR(3156)

Description

Application

3-Hydroxy-3-methylglutaryl-CoA reductase human has been used:

• in anti-HMGCR enzyme linked immunosorbent assay (ELISA)[1]
• as an antigen to measure anti-HMGCR antibodies by blot analysis[2]
• in HMG-CoA reductase activity assays[3]
• as an antigen to compare in-house antigen via sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE)/western blot[4]

Biochem/physiol Actions

3-Hydroxy-3-methylglutaryl-CoA reductase/HMGCR serves as a rate limiting enzyme in mevalonate pathway. It also controls the oncogenic Hippo transducers TAZ/YAP.[5] HMGCR also perform as a genetic modifier for Alzheimer′s disease (AD).[6]

Hydroxy-3-methylglutaryl-CoA reductase catalyzes the committed step in cholesterol biosynthesis.

Physical properties

Consists of the HMG CoA reductase catalytic domain fused to GST protein.

Unit Definition

One unit will convert 1.0 μmole of NADPH to NADP⁺ per minute at 37 °C.

Physical form

Supplied as a solution containing 250 μg protein in 50 mM Tris pH 7.5, with 5 mM DTT, 1:200 Protease Inhibitor Cocktail (Catalog Number P8340) and 50% (w/v) glycerol.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable