Both F9765 and F4767 are suitable for determining cholesterol in cells. The F4767, Filipin III, is purified from Filipin. As it has a higher purity, it may offer a higher signal-to-noise ratio resulting in less background. These product have not been compared in side by side testing. It may be helpful to view the peer-reviewed knowledge articles to help with the appropriate selection:
F9765
Filipin complex
powder, ≥70% (UV)
Synonym(s):
Filimarisin
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Product Name
Filipin complex from Streptomyces filipinensis, ≥70% (UV)
biological source
Streptomyces sp. (Streptomyces filipinensis)
Quality Level
Assay
≥70% (UV)
form
powder
solubility
methanol: 9.80-10.20 mg/mL, clear to hazy, yellow to very deep greenish-yellow
antibiotic activity spectrum
fungi
Mode of action
cell membrane | interferes
storage temp.
−20°C
InChI
1S/C35H58O11/c1-4-5-11-16-31(42)34-33(44)22-29(40)20-27(38)18-25(36)17-26(37)19-28(39)21-32(43)23(2)14-12-9-7-6-8-10-13-15-30(41)24(3)46-35(34)45/h6-10,12-15,24-34,36-44H,4-5,11,16-22H2,1-3H3/b7-6+,10-8+,12-9+,15-13+,23-14+/t24-,25+,26-,27+,28-,29+,30+,31-,32+,33+,34-/m1/s1
InChI key
IMQSIXYSKPIGPD-YQRUMEKGSA-N
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General description
Chemical structure: macrolide
Application
Biochem/physiol Actions
Filipin is a polyene macrolide antibiotic and antifungal. The antifungal mechanism of action is unclear but may be due to altering membrane permeability and associated functions via binding to membrane sterols. Filipin binds to membrane sterols such as cholesterol, and it both inhibits prion protein (PrP) endocytosis and causes the release of PrP from the plasma membrane.
Filipin is a polyene macrolide antibiotic and antifungal. The antifungal mechanism of action is unclear but may be due to altering membrane permeability. Filipin binds to model crystalline phase lipid bilayers containing cholesterol, and to liquid phase bilayers with or without cholesterol, in an orientation that forms membrane pores.It both inhibits prion protein (PrP) endocytosis and causes the release of PrP from the plasma membrane.
Other Notes
Filipin contains 8 isomeric components, with filipin III as the major component.
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Description
Pricing
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Which filipin is better to visualize cholesterol in hela cells by immunofluorescence - F9765 or F4767?
1 answer-
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What is the stability of filipin when dissolved in DMSO and stored at -20 degrees C (how long can it be stored like this)?
1 answer-
This product has not been tested for stability in solution, however, product SAE0088 is a prepared 5/mg/mL solution in DMSO. This product is stable for at least 2 years at -20 deg. C. Similar stability would be expected for the F9765. See the link below to the product information sheet on the SAE0088 solution:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/973/858/sae0088pis.pdfSee the link below for a sample Certificate of Analysis for the prepared solution which includes the retest date:
https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/SAE0088-1ML0000207634.pdfHelpful?
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the difference between Filipin products F9765 and F4767?
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The product F9765 consists of a filipin complex. It is composed of 8 isomeric components, of which filipin III is the major component in the complex. F4767 consists of filipin III alone and is considered to be a better choice for staining.
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What is the stability of stock solutions Product F9765, Filipin complex from Streptomyces filipinensis?
1 answer-
Solutions of filipin in aqueous or organic solvents are not considered to be stable and it is recommended that they be prepared fresh and not stored as stock solutions.
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How should Product F9765, Filipin complex from Streptomyces filipinensis, be solubilized?
1 answer-
This product has low solubility in aqueous buffers. It should initially be reconstituted in DMSO at 1 mg/mL and then diluted into saline or an aqueous buffer of choice.
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