Upon reconstitution, the molarity of glucose is 17.5 mM (3.15 g/L).
D8900
Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham
With L-glutamine and 15 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture
Synonym(s):
DMEM Hams F12, DME/F-12, 1:1 mixture
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About This Item
Pricing and availability is not currently available.
Quality Segment
form
powder
technique(s)
cell culture | mammalian: suitable
components
sodium pyruvate: 0.055 g/L
HEPES: 15 mM
NaHCO3: no
L-glutamine: yes
glucose: 3.15 g/L
phenol red: yes
shipped in
ambient
storage temp.
2-8°C
Application
Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham is suitable for the growth of a wide range of cell types in low serum conditions. It has been used:
- as a component of the culture medium for endometrial explants in vitro culture
- to immerse chitosan-gelatin-glycerol phosphate hydrogels for cytotoxicity evaluation
- to culture nucleus pulposus (NP) cells to culture human articular chondrocytes for evaluating the toxicity of baicalein
Preparation Note
Formulated to contain 15.6 grams of powder per liter of medium.
Supplement with 1.2 g/L sodium bicarbonate.
1 of 1
This Item | |||
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| form powder | form powder | form powder | form liquid |
| components sodium pyruvate: 0.055 g/L | components NaHCO3: no | components NaHCO3: no | components glucose: 3.15 g/L |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
| Quality Level 500 | Quality Level 500 | Quality Level 500 | Quality Level 400 |
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Storage Class
11 - Combustible Solids
wgk
WGK 1
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What is the molarity of glucose when reconstituted with water?
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