DPBS and other basal salt solutions are multi-component formulations. In total, this product requires 9.6 g to prepare 1L of a 1X solution. A molar concentration will not apply to the entire final product, only to the concentration of the individual components. See the link below for a formulation table for the DPBS products. The concentration of each component in these formulations are listed:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/354/091/d5773for.pdf
D5773
Dulbecco′s Phosphate Buffered Saline
Without calcium chloride, powder, suitable for cell culture
Synonym(s):
DPBS
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About This Item
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Application
Use this formulation of DPBS when tissue or cell dissociation or release is the objective. Also, suitable for studies that involve calcium metabolism.
Preparation Note
Formulated to contain 9.6 grams of powder per liter of medium.
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This Item | |||
|---|---|---|---|
| description Without calcium chloride, powder, suitable for cell culture | description liquid, sterile-filtered, with calcium, with magnesium, suitable for cell culture | description With MgCl2 and CaCl2, liquid, sterile-filtered, suitable for cell culture | description Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| Quality Level 500 | Quality Level 500 | Quality Level 400 | Quality Level 300 |
| form powder | form liquid | form liquid | form liquid |
| storage temp. 2-8°C | storage temp. room temp | storage temp. 2-8°C | storage temp. room temp |
DPBS ready-to-use solution for laboratories | 4′,4′′-Methylenebis(2-chloroacetanilide) procurement, 4′,4′′-Methylenebis(2-chloroacetanilide) ACS grade, 4′,4′′-Methylenebis(2-chloroacetanilide) chemical, 4′,4′′-Methylenebis(2-chloroacetanilide) for synthesis, 4′,4′′-Methylenebis(2-chloroacetanilide) reagent for titration, Sigma-Aldrich 4′,4′′-Methylenebis(2-chloroacetanilide) for research, 4′,4′′-Methylenebis(2-chloroacetanilide) safety data sheet (SDS), 4′,4′′-Methylenebis(2-chloroacetanilide) application in organic synthesis, 4′,4′′-Methylenebis(2-chloroacetanilide) technical data sheet, DPBS, Isotonic saline, cell washing solution, cell suspension solution, laboratory-grade 4′,4′′-Methylenebis(2-chloroacetanilide), high purity 4′,4′′-Methylenebis(2-chloroacetanilide), ordering 4′,4′′-Methylenebis(2-chloroacetanilide) in | DPBS, Isotonic saline, cell washing solution, cell suspension solution | d-PBS, Dulbeccos PBS, Dulbeccos Buffer, Phosphate Buffered Saline, buffered saline Dulbeccos, balanced salt solution (BSS), bss for mammalian cells, |
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Storage Class
13 - Non Combustible Solids
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Hello, it is my first time buying DPBS powder. May I know how much should I weigh to prepare 1M of solution? My product is D5773-10L. Thank you.
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