The purity of this material is determined by UV absorption at 260 nm. One milligram of DNA is equivalent to approximately 20 A260 units. This product is assigned a minimum A260 specification of 16. This information is lot specific and reported in the product Certificate of Analysis. Please see the link below to review a sample or lot specific certificate:
https://www.sigmaaldrich.com/US/en/product/sigma/d1501#product-documentation
D1501
Deoxyribonucleic acid sodium salt from calf thymus
Type I, fibers
Synonym(s):
ctDNA, DNA sodium salt from calf thymus, Thymonucleic acid sodium salt
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About This Item
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Application
Calf thymus DNA (CT-DNA) is a natural DNA widely used in studies of DNA binding anticancer agents and DNA binding agents that modulate DNA structure and function. Calf thymus DNA is also used in physicochemical studies of DNA behavior in solution.
Quality
"Highly Polymerized"
Physical properties
λmax 259 nm (100 mM phosphate buffer, pH 7.0).
DNA from calf thymus is 41.9 mole % G-C and 58.1 mole % A-T.1 An OD of 1.0 at 260 nm corresponds to ~ 50 μg of double-stranded DNA.
DNA from calf thymus is 41.9 mole % G-C and 58.1 mole % A-T.1 An OD of 1.0 at 260 nm corresponds to ~ 50 μg of double-stranded DNA.
Physical form
fibrous preparation
Preparation Note
This product is extracted using a method that causes shearing yielding a highly polymerized mixture of double and single stranded DNA . However, double stranded DNA is the predominant form.
This product is prepared from calf thymus tissue of unspecified gender.
Reconstitution
Solutions of DNA have been stored successfully for several months at 4 C, pH 7.5-8, in 10 mM Tris with 1 mM EDTA and without a bacteriostatic agent. At low concentrations (μg/ml) DNA tends to absorb onto the surfaces of plastic tubes. It is not recommended to store DNA in highly alkaline solutions since DNA tends to degrade at alkaline pH greater than 8.0.
This product can be dissolved at 2 mg/ml in water. To reduce further shearing of the DNA, no sonication or stirring should be used. Gentle inversion overnight at 0-4 °C is recommended to completely solubilize the DNA. The presence of 1 mM EDTA is recommended to prevent nucleases from degrading the DNA.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Protocols
Spectrophotometric assay at 260 nm measures nuclease S1 activity, vital for nucleic acid research, with defined enzyme unit criteria.
Spectrophotometric assay at 260 nm measures nuclease S1 activity, vital for nucleic acid research, with defined enzyme unit criteria.
Spectrophotometric assay at 260 nm measures nuclease S1 activity, vital for nucleic acid research, with defined enzyme unit criteria.
Spectrophotometric assay at 260 nm measures nuclease S1 activity, vital for nucleic acid research, with defined enzyme unit criteria.
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What is the purity of the chemical available?
1 answer-
Helpful?
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This product is tested in 15 mM NaCl with 1.5 mM Sodium citrate, pH 7.0 using a 1 cm light path. One mg of DNA is equivalent to 20 A260 units. Is the sodium citrate monobasic, dibasic or tribasic? thank you
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Sodium citrate tribasic dihydrate was used to test this product.
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How would you recommend soluting ctDNA for DNA determination? In Tris-EDTA (93302) or 15 mM NaCl and 1.5 mM Sodium Citrate? If we prepare the solution of 50 mg ctDNA + 50 ml Tris-EDTA and dilute it 1/20, would OD260 of 1.0 correspond to 50 ug/l? Thank you
1 answer-
This product is tested in 15 mM NaCl with 1.5 mM Sodium citrate, pH 7.0 using a 1 cm light path. One mg of DNA is equivalent to 20 A260 units. Using other buffers would not be recommended as the data could not be comparable to that reported in the product Certificate of Analysis. See the link below for a sample CofA:
https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/D1501-BULKSLBW1517.pdfHelpful?
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