Key Documents

View All Documentation

Dulbecco′s Modified Eagle′s Medium - high glucose

Name: Dulbecco′s Modified Eagle′s Medium - high glucose
Brand: SIGMA

HEPES Modification, With 4500 mg/L glucose, L-glutamine, and 25 mM HEPES, without sodium bicarbonate and pyruvate, powder, suitable for cell culture

Synonym(s):

DME, DMEM

Select a Size

Change View

Size/SKU	Availability	Price

About This Item

NACRES:

NA.75

UNSPSC Code:

12352207

Pricing and availability is not currently available.

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

form

powder

technique(s)

cell culture | mammalian: suitable

components

sodium pyruvate: no
NaHCO₃: no
phenol red: yes
HEPES: 25 mM
L-glutamine: yes
glucose: high

shipped in

ambient

storage temp.

2-8°C

Description

Application

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".

Preparation Note

Formulated to contain 17.3 grams of powder per liter of medium.

Supplement with 3.7 g/L sodium bicarbonate.

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item	D5648	D7777	D0422
Sigma-Aldrich D1152 Dulbecco′s Modified Eagle′s Medium - high glucose	Sigma-Aldrich D5648 Dulbecco′s Modified Eagle′s Medium - high glucose	Sigma-Aldrich D7777 Dulbecco′s Modified Eagle′s Medium - high glucose	Sigma-Aldrich D0422 Dulbecco′s Modified Eagle′s Medium - high glucose
description HEPES Modification, With 4500 mg/L glucose, L-glutamine, and 25 mM HEPES, without sodium bicarbonate and pyruvate, powder, suitable for cell culture	description With 4500 mg/L glucose and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture	description With 4500 mg/L glucose, L-glutamine, and sodium pyruvate, without sodium bicarbonate, powder, suitable for cell culture	description With 4500 mg/L glucose and sodium bicarbonate, without L-methionine, L-cystine and L-glutamine, liquid, sterile-filtered, suitable for cell culture
form powder	form powder	form powder	form liquid
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
components sodium pyruvate: no NaHCO₃: no phenol red: yes HEPES: 25 mM L-glutamine: yes glucose: high	components phenol red: yes L-glutamine: yes NaHCO₃: no HEPES: no glucose: high sodium pyruvate: no	components glucose: high sodium pyruvate: yes NaHCO₃: no HEPES: no phenol red: yes L-glutamine: yes	components glucose: high phenol red: yes L-glutamine: no sodium pyruvate: yes
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
shipped in ambient	shipped in ambient	shipped in ambient	shipped in ambient
classic media, classical media, FBS, glucose enriched	classic media, classical media, FBS, glucose enriched	classic media, classical media, FBS, glucose enriched	classic media, classical media, serum supplement, FBS

Still not finding the right product?

Explore all of our products under Dulbecco′s Modified Eagle′s Medium - high glucose

Storage Class

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.