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6-Carboxyfluorescein diacetate

Name: 6-Carboxyfluorescein diacetate
Brand: SIGMA

~95% purity (HPLC), powder

Synonym(s):

6-CFDA, 6-Carboxy-di-O-acetylfluorescein

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About This Item

Empirical Formula (Hill Notation):

C₂₅H₁₆O₉

CAS Number:

3348-03-6

Molecular Weight:

460.39

NACRES:

NA.47

PubChem Substance ID:

24892748

UNSPSC Code:

12171500

MDL number:

MFCD00036871

Beilstein/REAXYS Number:

67043

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Properties

Product Name

6-Carboxyfluorescein diacetate, ≥95% (HPLC)

Quality Level

100, 200

assay

≥95% (HPLC)

form

powder

color

white to yellow

solubility

chloroform/ethanol (1:1): 9.80-10.20 mg/mL, clear, colorless to light yellow

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

−20°C

SMILES string

CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5ccc(cc45)C(O)=O)c1

InChI

1S/C25H16O9/c1-12(26)31-15-4-7-18-21(10-15)33-22-11-16(32-13(2)27)5-8-19(22)25(18)20-9-14(23(28)29)3-6-17(20)24(30)34-25/h3-11H,1-2H3,(H,28,29)

InChI key

QMOGCCYGOPYYNT-UHFFFAOYSA-N

Description

Application

6-Carboxyfluorescein diacetate has been used to differentiate viable cells from apoptotic cells. Live cells that are not apoptotic accumulate 6-carboxyfluorescein but do not accumulate annexin.
Fluorescent wavelengths λex 492nm; λemm 517nm in 0.1M Tris, pH 8.0.

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1 of 1

This Item	C0662	87444	21879
Sigma-Aldrich C5041 6-Carboxyfluorescein diacetate	Sigma-Aldrich C0662 6-Carboxyfluorescein	Sigma-Aldrich 87444 5-Carboxy-fluorescein diacetate N-succinimidyl ester	Sigma-Aldrich 21879 5(6)-Carboxyfluorescein diacetate
color white to yellow	color dark yellow to dark orange	color -	color -
form powder	form powder	form powder	form powder
solubility chloroform/ethanol (1:1): 9.80-10.20 mg/mL, clear, colorless to light yellow	solubility 0.1 M NaOH: 9.80-10.20 mg/mL, clear to slightly hazy, orange	solubility DMF: soluble, DMSO: soluble	solubility DMSO: soluble
application(s) diagnostic assay manufacturing hematology histology	application(s) diagnostic assay manufacturing hematology histology	application(s) -	application(s) -
storage temp. −20°C	storage temp. 2-8°C	storage temp. −20°C	storage temp. 2-8°C
assay ≥95% (HPLC)	assay ≥96% (HPLC)	assay ≥95.0% (HPLC)	assay ≥90.0% (HPLC)

Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Rapid enumeration of physiologically active bacteria in purified water used in the pharmaceutical manufacturing process.

M Kawai et al.

Journal of applied microbiology, 86(3), 496-504 (1999-04-10)

Physiologically active bacteria in purified water used in the manufacturing process of pharmaceutical products were enumerated in situ. Bacteria with growth potential were enumerated using the micro-colony technique and direct viable counting (DVC), followed by 24 h of incubation in

Soluble MOG35-55/I-A(b) dimers ameliorate experimental autoimmune encephalomyelitis by reducing encephalitogenic T cells.

Yeli Gong et al.

PloS one, 7(10), e47435-e47435 (2012-10-19)

The MOG35-55 peptide-induced experimental autoimmune encephalomyelitis (EAE) model in C57BL/6 mice is a useful animal model to explore therapeutic approaches to T cell-mediated autoimmune diseases because the dominant T-cell epitope(s) have been defined. It is rational that antigen-specific immunosuppression can

Visualization of hepatobiliary excretory function by intravital multiphoton microscopy.

Yuan Liu et al.

Journal of biomedical optics, 12(1), 014014-014014 (2007-03-09)

Intravital imaging of hepatobiliary excretion is vital for elucidating liver metabolism. In this work, we describe a novel method to observe the intravital dynamics of the uptake, processing, and excretion of an organic anion, 6-carboxyfluorescein diacetate (6-CFDA) in the hepatobiliary

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Global Trade Item Number

SKU	GTIN
C5041-25MG	04061833501702
C5041-100MG	04061833501696

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