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Nancy-520

Name: Nancy-520
Brand: SIGMA

suitable for RT-PCR, ≥90.0% (HPCE)

Sustainability features

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About This Item

UNSPSC Code:

12171500

EC Number:

200-664-3

NACRES:

NA.32

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Properties

Quality Segment

100

assay

≥90.0% (HPCE)

form

liquid

mol wt

78.13 g/mol

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Sustainability features

color

red to very dark red

bp

189 °C/1,013 hPa

mp

18.4 °C

solubility

DMSO: soluble

density

1.104 g/cm³ at 20 °C

fluorescence

λ_ex 520 nm; λ_em 554 nm in TE buffer; DNA

greener alternative category

storage temp.

2-8°C

Description

General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product was Designed for Safer Chemistry

Application

Nancy-520 is a fluorescent stain for dsDNA on agarose electrophoresis gels, with higher sensitivity than ethidium bromide and an easy, fast, and robust staining procedure. It is provided as a 5000x stock solution (500 μl), which is sufficient for 50 agarose gels. The limit of detection is 0.5 ng/band of dsDNA.
In addition, Nancy-520 can be used to determine dsDNA concentrations in solution, with a linear range between 0 - 2 μg/ml of DNA. Nancy 520

Nancy-520 has been used successfully in PCR (Real time-PCR) applications.
Nancy 520

Greener alternative to Ethidium bromide.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

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This Item	43563	A9431	SBR00097
Sigma-Aldrich 01494 Nancy-520	Supelco 43563 Ancymidol	Sigma-Aldrich A9431 Ancymidol	Sigma-Aldrich SBR00097 Sancycline
description suitable for RT-PCR, ≥90.0% (HPCE)	description certified reference material, TraceCERT^®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland	description BioReagent, suitable for plant cell culture	description ≥95% (H-NMR), powder
fluorescence λ_ex 520 nm; λ_em 554 nm in TE buffer; DNA	fluorescence -	fluorescence -	fluorescence -
color red to very dark red	color -	color -	color yellow to brown
form liquid	form -	form powder	form powder
solubility DMSO: soluble	solubility -	solubility -	solubility -
Quality Level 100	Quality Level 300	Quality Level 200	Quality Level 100
assay ≥90.0% (HPCE)	assay -	assay -	assay ≥95% (H-NMR)

Storage Class

10 - Combustible liquids

WGK

WGK 2

Flash Point (°F)

188.6 °F - closed cup - Solvent

Flash Point (°C)

87 °C - closed cup - Solvent

PPE (personal protective equipment)

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

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