ABE48

Anti-NELF-E Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): Negative elongation factor E, NELF-E, RNA-binding protein RD
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: ICC, WB
• Citations: 2

Properties

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: rat, human, mouse
• species reactivity (predicted by homology): canine (based on 100% sequence homology), bovine (based on 100% sequence homology)
• technique(s): immunocytochemistry: suitable, western blot: suitable
• NCBI accession no.: NP_002895
• UniProt accession no.: P18615
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... NELFE(7936)

Description

General description

NELF-E (Negative elongation factor E) is a main component of NELF human transcription factor complex which associates with DRB sensitivity-inducing factor to stifle transcriptional elongation by RNA polymerase II. NELF-E is an RNA-binding protein that includes arginine-aspartic acid dipeptide repeats. NELF-E RNA-binding activity is essential for NELF to function.

~ 43 kDa observed. Uncharacterized bands may be due to post-translational modification.

Immunogen

KLH-conjugated linear peptide corresponding to human NELF-E.

Application

Anti-NELF-E Antibody is a Rabbit Polyclonal Antibody for detection of NELF-E also known as Negative elongation factor E, NELF-E, RNA-binding protein RD & has been validated in ICC & WB.

Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected NELF-E in NIH/3T3 and HeLa cells.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
L6 cell lysate

Evaluated by Western Blot in L6 cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected NELF-E on 10 µg of L6 cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1