ABC156

Anti-BAG family molecular chaperone regulator 3 (BAG3) Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): Bcl-2-associated athanogene 3, Bcl-2-binding protein Bis, Docking protein CAIR-1
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human
• species reactivity (predicted by homology): rhesus macaque (based on 100% sequence homology), mouse (based on 100% sequence homology), rat (based on 100% sequence homology), canine (based on 100% sequence homology)
• technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• NCBI accession no.: NP_004272
• UniProt accession no.: O95817
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... BAG3(9531)

Description

General description

Bcl2-associated athanogene 3 (BAG3), also known as CAIR-1 or Bis, belongs to a family of six evolutionarily conserved co-chaperone proteins that contain a characteristic C-terminal BAG domain. In most cell types, BAG3 is localized to the cytoplasm, where it regulates the heat shock protein 70 chaperone. BAG3 is activated in response to stressful stimuli, and plays a critical role in modulating multiple cellular processes, including apoptosis, autophagy, embryonic development, and actin rearrangement. Abnormal expression of BAG3 results cardiomyopathy dilated type 1HH.

Immunogen

KLH-conjugated linear peptide corresponding to human BAG3.

Application

Detect BAG family molecular chaperone regulator 3 (BAG3) using this Anti-BAG family molecular chaperone regulator 3 (BAG3) Antibody validated for use in Western Blotting, IHC(P).

Immunohistochemistry Analysis: A 1:500-1:1,000 dilution from a representative lot detected BAG3 in human cardiac and renal tissues.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Quality

Evaluated by Western Blot in DU145 cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected BAG3 in 10 µg of DU145 cell lysate.

Target description

~74/40 kDa observed. An uncharacterized band may appear at ~65 kDa in some lysates.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
DU145 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable