71408-M

Origami 2(DE3) Singles Competent Cells - Novagen

• Synonym(s): Competent E. coli Cells, Novagen Competent Cells
• UNSPSC Code: 41106202
• NACRES: NA.81

Properties

• manufacturer/tradename: Novagen^®
• Quality Level: 100
• storage condition: OK to freeze
• cell transformation: transformation efficiency: 2×10⁶ cfu/μg
• shipped in: dry ice
• storage temp.: −70°C

Description

General description

Origami 2 host strains are K-12 derivatives that have mutations in both the thioredoxin reductase (trxB) and glutathione reductase (gor) genes, which greatly enhance disulfide bond formation in the E. coli cytoplasm. The Origami 2 strains are kanamycin sensitive; like the original Origami strains, the gor mutation is still selected for by tetracycline. To reduce the possibility of disulfide bond formation between molecules, strains containing mutations in trxB and gor are recommended only for the expression of proteins that require disulfide bond formation for proper folding.

DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.

Genotype: Δ(ara-leu)7697 ΔlacX74 ΔphoA PvuII phoR araD139 ahpC galE galK rpsL F′[lac+ lacIq pro] (DE3) gor522::Tn10 trxB (StrR, TetR)

This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.

Components

• 11 or 22- Singles Competent Cells
• 2 or 4 × 2 ml- SOC Medium
• 10 µl- Test Plasmid

Warning

Toxicity: Multiple Toxicity Values, refer to MSDS

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany