06-873
Anti-Casein Kinase 2 Antibody
Upstate®, from rabbit
Synonym(s):
Anti-CK2A1, Anti-CKII, Anti-Cka1, Anti-Cka2, Anti-OCNDS
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse, human
manufacturer/tradename
Upstate®
technique(s)
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... CSN2(1447)
Specificity
Recognizes Casein Kinase 2.
Immunogen
peptide (C-SRARVYTDVNTHRPRE) corresponding to amino acids 7-22 of human Casein Kinase 2
Application
Anti-Casein Kinase 2 Antibody is an antibody against Casein Kinase 2 for use in WB.
Not recommended for IP.
Research Category
Signaling
Signaling
Research Sub Category
Developmental Signaling
Developmental Signaling
Quality
routinely evaluated by immunoblot on RIPA lysates from mouse 3T3/A31 fibroblasts or A431 cell lysates
Target description
44 kDa
Linkage
Replaces: 04-1129
Physical form
Format: Purified
Protein A Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
Protein A purified
Storage and Stability
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
Control
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Protein kinase CK2 ("casein kinase-2") and its implication in cell division and proliferation.
Pinna, L A and Meggio, F
Progress in Cell Cycle Research, 3, 77-97 (1997)
Hagen Kulbe et al.
Oncotarget, 7(13), 15648-15661 (2016-02-13)
We previously showed how key pathways in cancer-related inflammation and Notch signaling are part of an autocrine malignant cell network in ovarian cancer. This network, which we named the "TNF network", has paracrine actions within the tumor microenvironment, influencing angiogenesis
Proteomic analysis of proteins in PC12 cells before and after treatment with nerve growth factor: increased levels of a 43-kDa chromogranin B-derived fragment during neuronal differentiation.
C M Huang,H A Shui,Y T Wu,P W Chu,K G Lin,L S Kao,S T Chen
Brain Research Molecular Brain Research null
K Willert et al.
The EMBO journal, 16(11), 3089-3096 (1997-06-02)
The dishevelled (dsh) gene of Drosophila melanogaster encodes a phosphoprotein whose phosphorylation state is elevated by Wingless stimulation, suggesting that the phosphorylation of Dsh and the kinase(s) responsible for this phosphorylation are integral parts of the Wg signaling pathway. We
Wolfgang Bildl et al.
Neuron, 43(6), 847-858 (2004-09-15)
Small conductance Ca(2+)-activated K+ channels (SK channels) couple the membrane potential to fluctuations in intracellular Ca2+ concentration in many types of cells. SK channels are gated by Ca2+ ions via calmodulin that is constitutively bound to the intracellular C terminus
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