Common Markers and Small Molecule Inhibitors in Golgi Studies.

Common Markers and Small Molecule Inhibitors in Golgi Studies.

Methods in molecular biology (Clifton, N.J.) (2022-12-14)

Sarah Bui, Drew Stark, Jie Li, Jianchao Zhang, Yanzhuang Wang

RÉSUMÉ

In this chapter, we provide a detailed guide for the application of commonly used small molecules to study Golgi structure and function in vitro. Furthermore, we have curated a concise, validated list of endomembrane markers typically used in downstream assays to examine the consequent effect on the Golgi via microscopy and western blot after drug treatment. This chapter will be useful for researchers beginning their foray into the field of intracellular trafficking and Golgi biology.

MATÉRIAUX

Référence du produit

Marque

Description du produit

F0895

Sigma-Aldrich

FIBRONECTINE DE PLASMA HUMAIN, liquid, 0.1% (Solution), BioReagent, suitable for cell culture

T7765

Sigma-Aldrich

Tunicamycine from Streptomyces sp.

553210

Sigma-Aldrich

Rapamycin, ≥95% (HPLC), powder

C6628

Sigma-Aldrich

Chloroquine diphosphate salt, powder or crystals, 98.5-101.0% (EP)

E1383

Sigma-Aldrich

Etoposide, synthetic, 95.0-105.0%, powder

A7250

Sigma-Aldrich

N-Acetyl-L-cysteine, Sigma Grade, ≥99% (TLC), powder

569397

Sigma-Aldrich

Staurosporine, Streptomyces sp., Staurosporine, CAS 62996-74-, is a cell-permeable, potent, reversible, ATP-competitive inhibitor of protein kinases (IC₅₀ = 7, 20, 1.3, 0.7, & 8.5 nM for PKA, CAMK, MLCK, PKC, & PKG, respectively).

P0046

Sigma-Aldrich

Pyocyanin, from Pseudomonas aeruginosa, ≥98% (HPLC)

474787

Sigma-Aldrich

Z-Leu-Leu-Leu-al, Potent, reversible, and cell-permeable proteasome inhibitor (K_i = 4 nM).

A8456

Sigma-Aldrich

Fluorure de 4-(2-aminoéthyl)-benzènesulfonyle hydrochloride, ≥97.0% (HPLC)

344827

Sigma-Aldrich

Fucosyltransferase Inhibitor, 2F-Peracetyl-Fucose, 2F-Peracetyl-Fucose is a cell-permeable fluorinated fucose derivative that acts as an inhibitor of fucosyltransferases following its uptake and metabolic transformation into a GDP-fucose mimetic.

SML0506

Sigma-Aldrich

LY-411575, ≥97% (HPLC)

654380

Sigma-Aldrich

Tunicamycin, Streptomyces lysosuperficus

L5288

Sigma-Aldrich

Latrunculin B from Latruncula magnifica, ≥80% (HPLC), solid

196419

Millipore

BAPTA/AM, Membrane-permeable form of BAPTA.

11004638001

Roche

Chymostatin

L6785

Sigma-Aldrich

Lactacystin, ≥90% (HPLC)

203290

Sigma-Aldrich

Bisindolylmaleimide I, A highly selective, cell-permeable, and reversible protein kinase C (PKC) inhibitor (IC₅₀ = 10 nM) that is structurally similar to staurosporine.

I2764

Sigma-Aldrich

ML-7, powder

K1385

Sigma-Aldrich

KN-93, ≥98% (HPLC)

E3652

Sigma-Aldrich

Epoxomicin, ≥95% (HPLC), solid

11004646001

Roche

Antipain dihydrochloride, crystalline, suitable for cell culture, pkg of 10 mg

579053

Sigma-Aldrich

TAPI-1, InSolution, ≥97%, inhibits MMPs and TACE

565749

Sigma-Aldrich

β-Secretase Inhibitor II, The β-Secretase Inhibitor II, also referenced under CAS 263563-09-3, controls the biological activity of β-Secretase. This small molecule/inhibitor is primarily used for Neuroscience applications.

565771

Sigma-Aldrich

γ-Secretase Inhibitor X, InSolution, ≥90%, 1 mM

M3694

Sigma-Aldrich

Anti-α1,2-Mannosidase IA antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution