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66905-U

Supelco

BIOshell A160 Peptide C18 (2.7 μm) HPLC Columns

L × I.D. 15 cm × 2.1 mm, HPLC Column

Synonyme(s) :

Superficially Porous Wide Pore C18 UHPLC Column

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About This Item

Code UNSPSC :
41115700
Nomenclature NACRES :
SB.52

product name

BIOshell A160 Peptide C18, 2.7 μm HPLC Column, 2.7 μm particle size, L × I.D. 15 cm × 2.1 mm

Matériaux

stainless steel hardware

Niveau de qualité

Agence

suitable for USP L1

Description

Shell thickness (0.5 μm)
Solid Core (1.7 μm)

Gamme de produits

BIOshell
BIOshell

Caractéristiques

endcapped: no

Classe(s) chimique(s) de l'analyte

peptides
peptides

Fabricant/nom de marque

BIOshell
BIOshell

Conditionnement

1 ea of

Ampleur du marquage

4.6% carbon loading

Paramètres

600 bar max. pressure
90 °C max. temp.

Technique(s)

HPLC: suitable
HPLC: suitable
LC/MS: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
UHPLC: suitable

L × D.I.

15 cm × 2.1 mm

Superficie

90 m2/g

Couverture de surface

2.2 μmol/m2

Matrice

spherical silica particle platform
superficially porous particle
superficially porous particle

Groupe de la matrice active

C18 (octadecyl) bonding phase, diisobutyloctadecyl

Taille des particules

2.7 μm
2.7 μm

Dimension de pores

160 Å

operating pH range

1-8

Technique de séparation

reversed phase
reversed phase

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Description générale

BIOshell 160 Å Peptide C18 columns are specifically engineered to provide efficient separation of peptides as well as small proteins. These columns contain advanced Fused-Core particles with pores strategically sized to 160 Å to enable optimized peptide diffusion. This attribute makes these columns an excellent choice for peptide mapping. Additionally, the sterically-protected C18 ligands provide extra stability allowing the columns to be used at an extended pH range (2-9) and high temperatures (up to 90 °C). This greatly expands the application range for the separation of biomolecules.

Application

BIOshell A160 Peptide C18, 2.7 μm HPLC Column, a non-end capped column that has superficially porous particle with C18 alkyl ligand as the stationary phase, is used mainly in the separation of peptides at a good flow rate.

Caractéristiques et avantages

BIOshell A160 Peptide C18, 2.7 μm HPLC Columns are compatible both with UHPLC and HPLC systems. They are specifically engineered for the fast and high-resolution seperation of biomolecules. These columns operate at high flow rates and have 40% higher efficiency than columns with fully porous particles of the same size. They are stable at high temperatures, which increases the throughput and enhances the peak shape.

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Informations légales

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Collagen-derived X-Hyp-Gly-type tripeptides promote differentiation of MC3T3-E1 pre-osteoblasts
Taga Y, et al.
Journal of functional foods, 46, 456-462 (2018)
Jennifer K Field et al.
Journal of chromatography. A, 1622, 461093-461093 (2020-04-29)
The Peptide RPC Column Characterisation Protocol was applied to 38 stationary phases, varying in ligand chemistry, base silica, end capping and pore size, which are suitable for the analysis of peptides. The protocol at low and intermediate pH is based

Articles

This application note compares three different column chemistries from the BIOshell™ line of columns with superficially porous particles to evaluate their performance in peptide mapping.

This application note compares three different column chemistries from the BIOshell™ line of columns with superficially porous particles to evaluate their performance in peptide mapping.

This application note compares three different column chemistries from the BIOshell™ line of columns with superficially porous particles to evaluate their performance in peptide mapping.

This application note compares three different column chemistries from the BIOshell™ line of columns with superficially porous particles to evaluate their performance in peptide mapping.

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Protocoles

Chromatographic technique identifies insulin variants efficiently, aiding in pharmacokinetics.

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Chromatographic technique identifies insulin variants efficiently, aiding in pharmacokinetics.

Chromatographic technique identifies insulin variants efficiently, aiding in pharmacokinetics.

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