ZMS1212

Anti-p-Ser/Thr-Pro MPM-2 Antibody, clone MPM-2 ZooMAb^® Mouse Monoclonal

recombinant, expressed in HEK 293 cells

• Synonyme(s) : Anti-NKX2-1 Antibody, clone 1H5-H2 ZooMAb^® Rabbit Monoclonal, phospho-MPM2 (Ser/Thr)-Pro,
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.43

Propriétés

• Source biologique: mouse
• Niveau de qualité: 200
• Produit recombinant: expressed in HEK 293 cells
• Conjugué: unconjugated
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: MPM-2, recombinant monoclonal
• Description: recombinant, expressed in HEK 293 cells
• Gamme de produits: ZooMAb^® learn more
• Forme: lyophilized
• Poids mol.: calculated mol wt Variable kDa; observed mol wt ~50-180 kDa
• Produit purifié par: using protein G
• Espèces réactives: human
• Réactivité de l'espèce (prédite par homologie): all
• Conditionnement: antibody small pack of 25
• Caractéristiques du produit alternatif plus écologique: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• Validation améliorée: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• Technique(s): immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotype: IgG1
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C

Description

Description générale

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Spécificité

Clone MPM-2 is a ZooMAb^® mouse recombinant monoclonal antibody that specifically detects phosphorylated serines/threonines when followed by a proline in mitotic proteins.

Immunogène

Mitotic HeLa cell cytosolic lysate.

Application

Quality Control Testing

Evaluated by Immunohistochemistry (Paraffin) in Human skin tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution of this antibody detected phospho-S/T-Pro MPM-2 in Human skin tissue sections.

Tested Applications

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected phospho-S/T-Pro MPM-2 in Human prostate cancer tissue sections

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected phospho-S/T-Pro MPM-2 in HeLa cells.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected phospho-S/T-Pro MPM-2 in HeLa cells treated with colcemid.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Description de la cible

Upon entry into Mitotic (M) phase of cell cycle, many proteins are phosphorylated either directly or indirectly by M-phase-promoting factor (MPF). The reversible phosphorylation of proteins regulates transitions between various phases of the cell cycle and the cytoarchitectural changes necessary for cell division. One such group of proteins is the family of MAP kinases that play critical roles in cell cycle control. They mediate intracellular signal transmission in response to external stimuli. p42MAP kinase requires phosphorylation on both threonine 183 and tyrosine 185 for its full activation. This MPM-2 recombinant monoclonal antibody binds to a phospho amino acid-containing epitopes present on several proteins of M-phase eukaryotic cells. It recognizes phosphorylated serine/threonine epitope that is followed by a proline residue. It is reported to recognizes the activated form of p42 MAP kinase. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, o

Forme physique

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stockage et stabilité

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Informations légales

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable