Y2001

Yeast Synthetic Drop-out Medium Supplements

without histidine, leucine, tryptophan and uracil

• Synonyme(s) : Synthetic drop-out medium for yeast
• Numéro MDL: MFCD01095679
• Code UNSPSC : 12000000
• Nomenclature NACRES : ND.02

Propriétés

• Niveau de qualité: 100
• Forme: powder
• Application(s): food and beverages

Description

Application

The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. Sigma′s Yeast Synthetic Drop-Out Media Supplements create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs.

Composants

The supplements contain all of the following components except as indicated:
Amino acids: All standard amino acids except for histidine, leucine, tryptophan and uracil. Each amino acid is present at a concentration of 76 mg/L.
Other nutrients: Adenine (18 mg/L), inositol (76 mg/L), p-aminobenzoic acid (8 mg/L)

Autres remarques

Mixtures of amino acids and other nutrients to be added to Yeast Nitrogen Base Without Amino Acids.

Quantité

1.4 g will supplement 1 liter of medium.

Informations sur la sécurité

• Code de la classe de stockage: 13 - Non Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)