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Sigma-Aldrich

Extract-N-Amp™ Plant PCR Kit

sufficient for 1000 extractions, sufficient for 1000 amplifications

Synonyme(s) :

Plant direct PCR kit

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About This Item

Code UNSPSC :
41106303
Nomenclature NACRES :
NA.55

Utilisation

sufficient for 1000 amplifications
 sufficient for 1000 extractions

Caractéristiques

hotstart

Technique(s)

PCR: suitable

Couleur

colorless

Conditions d'expédition

wet ice

Température de stockage

−20°C

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Description générale

The Extract-N-Amp™ Plant PCR Kits are intended for direct PCR (polymerase chain reaction). This kit contains all the reagents necessary to rapidly extract genomic DNA from plant leaves and amplify targets of interest by PCR. It is a novel Extraction Solution that eliminates the need for conventional freezing of plant tissues.

Application

Extract-N-Amp™ Plant PCR Kit has been used for DNA extraction from fresh mushrooms.
Extract-N-Amp™ Plant PCR Kit is suitable for use in genotyping.

It has been used forDNA extraction from fresh mushrooms.

Caractéristiques et avantages

  • Plant genomic DNA can be extracted for PCR in under 15 minutes using a method.
  • No freezing, mechanical disruption, organic extraction, column purification or precipitation is necessary
  • A specially formulated PCR ReadyMix is provided for use with the extracted DNA
  • The Hot Start antibody ensures highly specific PCR amplification of genomic DNA
  • Suitable for high-throughput plant genetic analysis.
  • Lengthy enzymatic digestions are not required, saving time and effort.
  • The extracted DNA remains stable at 4 °C for atleast 6 months.

Composants

Extract-N-Amp™ Plant PCR Kits contain Dilution solution, Extraction solution, and Extract-N-Amp PCR Reaction Mix (2X) (buffer, salts, dNTPs, Taq polymerase, and JumpStart Taq antibody)

Autres remarques

For additional information, please see www.sigma-aldrich.com/extract-n-amp.
The Extract-N-Amp Plant PCR Kits are for laboratory use only. Not for drug, household, or other uses.

Informations légales

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

XNAP

Kit de PCR REDExtract-N-Amp™ Plant, sufficient for 100 extractions, sufficient for 100 amplifications

XNAPE

Kit de PCR REDExtract-N-Amp™ Plant, sufficient for 100 extractions, sufficient for 500 amplifications

XNAP2

Extract-N-Amp™ Plant PCR Kit, sufficient for 100 extractions, sufficient for 100 amplifications

XNAPR

Kit de PCR REDExtract-N-Amp™ Plant, sufficient for 1000 extractions, sufficient for 1000 amplifications

XNAPS

Kit de PCR REDExtract-N-Amp™ Plant, sufficient for 10 extractions, sufficient for 10 amplifications

R4775

ReadyMix PCR REDExtract-N-Amp™™, Ready-to-use 2X PCR Master Mix with Loading Dye

E3004

ReadyMix pour PCR Extract-N-Amp™™, Amplifications to support Extract-N-Amp Plant and Extract-N-Amp Tissue

E8155

Extract-N-Amp™ PCR Diluent, colorless, storage temp.:2-8°C

D5688

Dilution Solution, Extract-N-Amp Plant Dilution Solution

XNAPG

SYBR® Green Extract-N-Amp™ Plant PCR Kit, sufficient for 100 preparations

S4320

SYBR® Green Extract-N-Amp™ PCR ReadyMix™, Amplifications to support Extract-N-Amp Plant and Extract-N-Amp Tissue

E0781

Extract-N-Amp™ PCR ReadyMix™ with qPCR Reference dye, 125 mL sufficient for 10000 amplifications, hotstart, dNTPs included

Pictogrammes

Exclamation markEnvironment
GHS07,GHS09

Mention d'avertissement

Warning

Mentions de danger

H315,H317,H319,H411

Classification des risques

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Alison Heffer et al.
EvoDevo, 2(1), 7-7 (2011-03-03)
Tremendous progress has been made in the field of evo-devo through comparisons of related genes from diverse taxa. While the vast number of species in nature precludes a complete analysis of the molecular evolution of even one single gene family
M Riahi et al.
Genetics and molecular research : GMR, 9(3), 1334-1342 (2010-07-21)
Three DNA extraction protocols were compared for their ability to yield DNA from the leaves of herbarium specimens of nine species from nine genera of the Papilionoideae. We tested two protocols that use classic procedures for lysis and purification with
Jian-Feng Li et al.
Plant methods, 6(1), 1-1 (2010-02-26)
Research in plant molecular biology involves DNA purification on a daily basis. Although different commercial kits enable convenient extraction of high-quality DNA from E. coli cells, PCR and agarose gel samples as well as plant tissues, each kit is designed
Matthew E Smith et al.
PloS one, 8(1), e55160-e55160 (2013-02-06)
Ectomycorrhizal (ECM) plants and fungi can be diverse and abundant in certain tropical ecosystems. For example, the primarily paleotropical ECM plant family Dipterocarpaceae is one of the most speciose and ecologically important tree families in Southeast Asia. Pakaraimaea dipterocarpacea is
Dionne N Shepherd et al.
Journal of virological methods, 149(1), 97-102 (2008-02-19)
A high-throughput method of isolating and cloning geminivirus genomes from dried plant material, by combining an Extract-n-Amp-based DNA isolation technique with rolling circle amplification (RCA) of viral DNA, is presented. Using this method an attempt was made to isolate and
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Articles

Extract-N-Amp™ Plant PCR Kits

Extract-N-Amp kits extract and amplify genomic DNA, optimized for plant tissue without inhibition concerns.

Extract-N-Amp™ Plant PCR Kits

Extract-N-Amp kits extract and amplify genomic DNA, optimized for plant tissue without inhibition concerns.

Extract-N-Amp™ Plant PCR Kits

Extract-N-Amp kits extract and amplify genomic DNA, optimized for plant tissue without inhibition concerns.

Extract-N-Amp™ Plant PCR Kits

Extract-N-Amp kits extract and amplify genomic DNA, optimized for plant tissue without inhibition concerns.

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Protocoles

Extract-N-Amp™ Plant PCR Kits Protocol

The Extract-N-Amp™ Plant PCR Kits contain all of the reagents required to rapidly extract and amplify genomic DNA from plant leaves.

Extract-N-Amp™ Plant PCR Kits Protocol

The Extract-N-Amp™ Plant PCR Kits contain all of the reagents required to rapidly extract and amplify genomic DNA from plant leaves.

Extract-N-Amp™ Plant PCR Kits Protocol

The Extract-N-Amp™ Plant PCR Kits contain all of the reagents required to rapidly extract and amplify genomic DNA from plant leaves.

Extract-N-Amp™ Plant PCR Kits Protocol

The Extract-N-Amp™ Plant PCR Kits contain all of the reagents required to rapidly extract and amplify genomic DNA from plant leaves.

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