V8137

Anti-V5 antibody produced in rabbit

IgG fraction of antiserum

• Synonyme(s) : Rabbit Anti-V5
• UNSPSC Code: 12352203
• NACRES: NA.56
• MDL number: MFCD03456076
• Conjugate: unconjugated
• Clone: polyclonal
• Application: ICC, IP, WB
• Citations: 121

Propriétés

• biological source: rabbit
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• technique(s): immunocytochemistry: 5 μg/mL using V5 tagged fusion protein in methanol-acetone fixed transiently transfected 293T cells, immunoprecipitation (IP): 5 μg/mL using V5 tagged fusion protein from extracts of transfected cells, western blot: 2.5 μg/mL using V5 tagged fusion protein expressed in whole extracts of transfected cells
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

V5 is an amino coterminal protein consisting of a C-terminus cysteine-rich region that resembles a cysteine-rich metal binding domain. It is encoded by the "P" gene of the paramyxovirus SV5.
The rabbit Anti-V5 synthetic peptide antibody reacts with V5 tagged recombinant fusion proteins expressed in transfected mammalian cells and from in vitro translation.

Application

Anti-V5 antibody produced in rabbit has been used in:

• immunocytochemistry
• immunoprecipitation
• western blot

Biochem/physiol Actions

Recognizes V5 Tag (GKPIPNPLLGLDST) fusion proteins

Physical form

Solution in 0.01M phosphate buffered saline, pH 7.4 containing 15 mM sodium azide.

Preparation Note

Store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in “frost-free” freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Classe de stockage: 10 - Combustible liquids
• Que se passe-t-il ?: WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• EPI (équipement de protection individuelle): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)