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T9028

Sigma-Aldrich

Monoclonal Anti-Tubulin, Tyrosine antibody produced in mouse

clone TUB-1A2, ascites fluid

Synonyme(s) :

Anti-CDCBM6, Anti-CSCSC1, Anti-M40, Anti-OK/SW-cl.56, Anti-TUBB1, Anti-TUBB5

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About This Item

Numéro MDL:
MFCD00164753
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

200

Conjugué

unconjugated

Forme d'anticorps

ascites fluid

Type de produit anticorps

primary antibodies

Clone

TUB-1A2, monoclonal

Contient

15 mM sodium azide

Espèces réactives

human, plant, animal

Technique(s)

indirect immunofluorescence: 1:800 using cultured chicken fibroblasts
microarray: suitable
western blot: suitable

Isotype

IgG3

Numéro d'accès UniProt

P07437

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... TUBA4A(7277) , TUBB(203068)

Description générale

Monoclonal Anti-Tyrosine Tubulin (mouse IgG3 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Tubulin as a cylindrical filamentous structure and is present in almost all eukaryotic cells. Tubulin is a heterodimer which consists of α-tubulin and β-tubulin; both subunits have a molecular weight of 55 kDa and share considerable homology.
The intracellular cylindrical filamentous structure, microtubules is mainly made up of tubulin and is present in all eukaryotic cells. Monoclonal Anti-Tyrosine antibody can be used in immunocytochemical localization of tyrosinated α tubulin by indirect immunofluorescence labelling. Monoclonal Anti-Tyrosine antibody reacts specifically with tyrosine tubulin of bovine brain, African Green Monkey kidney cells (Vero), dog kidney (MDCK), marsupial kidney (Potoroo, PtK2), mouse pituitary tumour (AtT-20), yeast, and Xenopus.

Spécificité

The antibody reacts against tubulin′s C-terminal tyrosine in immunoblotting assays and may be used for localization of this epitope in cultured cells or tissue sections.

Immunogène

peptide containing the carboxy-terminal amino acids of α-tubulin

Application

Monoclonal Anti-Tyrosine Tubulin has been used in:
  • indirect immunofluorescent labelling
  • immunoblotting technique
  • immunocytochemical staining

Actions biochimiques/physiologiques

Tubulin acts as a major building block of microtubules. Tubulin tyrosinylation is involved in the assembly status of tubulin. A specific tubulinyl tyrosine carboxypeptidase removes the terminal tyrosine to yield an α-tubulin terminating in a glutamic acid residue while another enzyme modifies the α-tubulin by addition of tyrosine to the carboxy terminus to offer a potential cycle of tyrosine addition and loss.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Description
Tarif

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

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Consulter la Bibliothèque de documents

Yasuharu Takaku et al.
Scientific reports, 4, 3573-3573 (2014-01-08)
Nerve cells and spontaneous coordinated behavior first appeared near the base of animal evolution in the common ancestor of cnidarians and bilaterians. Experiments on the cnidarian Hydra have demonstrated that nerve cells are essential for this behavior, although nerve cells
On some features of embryonic development and metamorphosis of Aurelia aurita (Cnidaria, Scyphozoa)
Mayorova TD, et al.
Russian journal of developmental biology, 43(5), 271-285 (2012)
Malkolm Graffe et al.
The Journal of general physiology, 146(1), 109-117 (2015-07-01)
A set of bipolar cells in the retina of goldfish contains giant synaptic terminals that can be over 10 µm in diameter. Hundreds of thousands of synaptic vesicles fill these terminals and engage in continuous rounds of exocytosis. How the
Microtubule Structures
Molecular Cell Biology. 4th edition. (2000)
Nagayasu Nakanishi et al.
BMC biology, 12, 26-26 (2014-04-01)
The evolutionary origin of gastrulation--defined as a morphogenetic event that leads to the establishment of germ layers--remains a vexing question. Central to this debate is the evolutionary relationship between the cell layers of sponges (poriferans) and eumetazoan germ layers. Despite
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Articles

Antibodies to Tubulins, including Centrosome-Associated ε-Tubulin

Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of α- and β-tubulin. In addition to α-and β-tubulin, several other tubulins have been identified, bringing the number of distinct tubulin classes to seven.

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