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For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
T8787
Triton™ X-100
Molecular Biology
Synonyme(s) :
t-Octylphénoxypolyéthoxyéthanol, Éther tert-octylphénylique du polyéthylène glycol
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A propos de cet article
biological source
synthetic (organic)
grade
Molecular Biology
description
non-ionic
form
liquid
mol wt
micellar avg mol wt 80,000, average mol wt 625
aggregation number
100-155
technique(s)
protein purification: suitable, protein quantification: suitable, western blot: suitable
impurities
<1.00% water (Karl Fischer)
pH
9.7
CMC
0.2-0.9 mM (20-25°C)
mp
6 °C
transition temp
cloud point 65 °C, pour point ~7 °C
solubility
water: 0.1 mL/mL, clear to slightly hazy, colorless to faintly yellow
density
1.06 g/mL at 25 °C (lit.)
cation traces
Fe: <5 ppm, K: <0.05%, Na: <0.1%, heavy metals: <5 ppm
HLB
13.5
application(s)
hematology
histology
foreign activity
DNase and RNase, none detected
storage temp.
room temp
SMILES string
CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1
InChI
1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3
InChI key
HNLXNOZHXNSSPN-UHFFFAOYSA-N
General description
Application
- pour marquer les rétines montées à plat par immunohistochimie[1]
- pour des analyses d′ADN cellulaire et essais d′annexine V sur des cellules en suspension, avec un tampon phosphate salin (PBS) glacé
- pour perméabiliser les cellules dans les études de microscopie à immunofluorescence[2]
- comme contrôle positif dans un essai LDH pour déterminer l′intégrité de la membrane cellulaire[3]
- pour estimer l′activité de lipases dans le plasma après traitement par héparine en utilisant la procédure radio-enzymatique modifiée de Belfrage et Vaughan
- pour la préparation d′extraits de protéines de membrane externe
- comme composant du tampon d′extraction avec Tris-HCl, NaCl, CaCl2, ZnCl2, Brij 35 pour l′homogénéisation de cellules pulmonaires de souris
- pour le traitement de coupes de tissus en vue du marquage par immunofluorescence
Biochem/physiol Actions
Features and Benefits
- Produit idéal pour les applications de recherche sensibles en biologie moléculaire.
- Testé pour garantir l'absence de DNases et de RNases.
- Détergent non ionique non dénaturant.
- Agent mouillant et émulsifiant performant.
- Tensioactif extrêmement polyvalent.
Other Notes
Legal Information
1 of 1
Cet article | |||
|---|---|---|---|
| technique(s) protein purification: suitable, western blot: suitable, protein quantification: suitable | technique(s) - | technique(s) - | technique(s) - |
| grade Molecular Biology | grade laboratory grade | grade - | grade laboratory grade |
| form liquid | form liquid | form liquid | form - |
| aggregation number 100-155 | aggregation number 100-155 | aggregation number 100-155 | aggregation number - |
| application(s) hematology | application(s) advanced drug delivery | application(s) - | application(s) - |
| storage temp. room temp | storage temp. - | storage temp. 15-25°C | storage temp. - |
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signalword
Danger
Classe de stockage
10 - Combustible liquids
wgk
WGK 3
flash_point_f
483.8 °F - closed cup
flash_point_c
251 °C - closed cup
ppe
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - ED ENV 1 - Eye Dam. 1 - Skin Irrit. 2
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How can I determine the shelf life / expiration / retest date of this product?
1 answer-
Helpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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What is the concentration of Triton X-1000 T8787?
1 answer-
This product is considered a neat or pure liquid at 100% active ingredient. The maximum allowable water content is 1%. This value is lot specific and reported on the Certificate of Analysis.
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Hello, I am wondering how could I find the purity of the Triton X-100 in your products. Your help and advice is appreciated. Kind regards, Soheil
1 answer-
This product is 100 % active ingredient. The maximum allowable water content is 1 % for this product.
Please see the link below to review a sample Certificate of Analysis:
https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/T8787-VAR0000267901.pdfHelpful?
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Can pure Triton X-100 be stored at and remain stable at less than 4C?
1 answer-
This product has been subjected, in the pure form, to temperatures at or near the pour point (1 °C) with no degradation. At lower temperatures, the material may become viscous and require gentle warming to ensure homogeneity. In dilute solutions, such as cell lysates or protein samples, Triton X-100 is an excellent freeze/thaw stabilizer.
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What form is this in?
1 answer-
This product is in liquid form at room temperature (melting point is 6 deg C) as listed in the "Properties" section of the product detail page.
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