T5391

apo-Transferrine human

γ-irradiated, powder, BioXtra, suitable for cell culture, ≥98%

• Synonyme(s) : Sidérophiline, Transferrine humaine
• Numéro CAS: 11096-37-0
• UNSPSC Code: 12352202
• EC Number: 234-318-8
• NACRES: NA.75
• MDL number: MFCD00130536
• eCl@ss: 34058011

Propriétés

• Quality Segment: 200
• sterility: γ-irradiated
• product line: BioXtra
• assay: ≥98%
• form: powder
• mol wt: 76-81 kDa
• concentration: ~25 mM
• technique(s): cell culture | mammalian: suitable
• impurities: HIV and HBsAg, source material tested negative, iron, essentially free
• solubility: H₂O: 50 mg/mL
• UniProt accession no.: P02787
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... TF(7018)

Description

General description

apo-Transferrin (aTF) human can be supplemented with iron or used to bind free iron present in media. It can be used to transport iron into mammalian cells.

Application

apo-Transferrin human has been used as a supplement:

• in Dulbecco′s modified Eagle medium/HamF12 (DMEM/F-12) to culture human adrenocortical carcinoma cells
• in DMEM/F-12 to culture neural precursor cells from mice
• in collecting medium NCSU23 to culture preantral follicles from porcine ovaries

Biochem/physiol Actions

apo-Transferrin (aTF) is an endogenous iron chelator and shows protective effects in the host by scavenging free iron and preventing the formation of toxic reactive oxygen species (ROS). It shows a therapeutic effect against thalassemia. After the release of iron, receptor-bound apo-transferrin reaches the cell surface instead of being transported to lysosomes for degradation. The cell surface has a neutral pH that promotes the release of apo-transferrin from its receptor, where it can again circulate and bind additional iron to undergo further rounds of iron delivery to cells.

Other Notes

Protéine de transport du fer du type non-hème.

Disclaimer

Ce produit, destiné à la recherche scientifique, est soumis à une réglementation spécifique en France, y compris pour les activités d′importation et d′exportation (Article L 1211-1 alinéa 2 du Code de la Santé Publique). L′acheteur (c′est-à-dire l′utilisateur FINAL) est tenu d′obtenir une autorisation d′importation auprès du ministère français de la recherche, mentionné à l′article L1245-5-1 II du Code de la Santé Publique. En commandant ce produit, vous confirmez détenir l′autorisation d′importation requise.

Informations sur la sécurité

• Classe de stockage: 11 - Combustible Solids
• wgk: WGK 3