T5300

Transforming Growth Factor-β2 from porcine platelets

≥97% (SDS-PAGE and N-terminal analysis), recombinant, powder, suitable for cell culture

• Synonyme(s) : TGF-β2
• Numéro MDL: MFCD00163957
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.77

Propriétés

• product name: Transforming Growth Factor-β2 from porcine platelets, powder, suitable for cell culture
• Source biologique: Porcine (Platelets)
• Niveau de qualité: 200
• Pureté: ≥97% (SDS-PAGE and N-terminal analysis)
• Forme: powder
• Puissance: 0.05-0.5 ng/mL ED₅₀/EC₅₀
• Poids mol.: protein 25 kDa
• Conditionnement: pkg of 1 μg
• Conditions de stockage: avoid repeated freeze/thaw cycles
• Technique(s): cell culture | mammalian: suitable
• Impuretés: endotoxin, tested
• Numéro d'accès UniProt: F1S9L0
• Température de stockage: −20°C
• Informations sur le gène: pig ... TGFB2(397084)

Description

Description générale

TGF-β2 (transforming growth factor-β2) belongs to the TGF-β superfamily and the gene is mapped to pig (Sus scrofa) chromosome 10p16.

Actions biochimiques/physiologiques

TGF-β2 is responsible for maintaining immune homeostasis. TGF-β2 promotes cellular growth, differentiation and survival. TGF-β2 controls lymphocyte proliferation, apoptosis, hematopoiesis, and embryogenesis. TGF-β2 is a tumor suppress or in the early stages of carcinogenesis, but in the later stages acts as a tumor promoter by inducing epithelial-mesenchymal transition and stimulating angiogenesis. TGF-β2 prevents NK (natural killer) cells growth as well as B and T cell proliferation. TGF-β2 also greatly inhibits the growth of both normal and transformed epithelial. The growth of lymphoid, fibroblast and keratinocyte cells are also known to be hinder by TGF-β2. TGF-β2 controls intestinal homeostasis by functioning in synergy with the bacterial lipopolysaccharide and thereby maintain interleukin-8.

TGF-β2, like TGF-β1, is produced by many cell types and reported to be most concentrated in mammalian platelets.

TGF-β2 belongs to the TGF-β superfamily. TGF-β2 is important for immune homeostasis by balancing lymphocyte proliferation, apoptosis, hematopoiesis, and embryogenesis. TGF-β2 is crucial in cell growth, differentiation, and survival. TGF-β2 is a strong growth inhibitor for normal and transformed epithelial, lymphoid, fibroblast, and keratinocyte cells. TGF-β2 is a tumor suppress or in the early stages of carcinogenesis, but in the later stages acts as a tumor promoter by inducing epithelial-mesenchymal transition and stimulating angiogenesis. TGF-β2 inhibits NK cells growth as well as B and T cell proliferation.

TGF-β2, like TGF-β1, is produced by many cell types and reported to be most concentrated in mammalian platelets.

Forme physique

Lyophilized from a 0.2 μm filtered solution in 25% acetonitrile and 0.1% trifluoroacetic acid containing 0.05 mg bovine serum albumin.

Remarque sur l'analyse

The biological activity is measured by its ability to inhibit the IL-4-dependent proliferation of mouse HT-2 cells.

The biological activity of porcine TGF-β2 was tested in culture by measuring its ability to inhibit thymidine incorporation in the IL-4 dependent mouse T-helper cell line HT-2. The EC50 is defined as the effective concentration of growth factor that elicits 50% inhibition of cell growth in a cell based bioassay.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)