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Key Documents

T2400

Sigma-Aldrich

Tropomyosin from porcine muscle

lyophilized powder (contains Tris buffer salts)

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About This Item

Numéro CAS:
Numéro MDL:
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.77

Source biologique

Porcine muscle

Niveau de qualité

Pureté

≥70% protein basis (biuret)

Forme

lyophilized powder (contains Tris buffer salts)

Technique(s)

electrophoresis: suitable

Température de stockage

−20°C

Description générale

Tropomyosin is a member of the actin filament binding protein family. It is a ubiquitous protein present in animals and fungi. Tropomyosin is made up of two α-helical chains arranged as a coiled-coil. It exists over twenty isoforms, in different eukaryotic cells like skeletal, cardiac, and smooth muscles, brain, platelets, fibroblasts, platelets, and other non-muscle cells.

Application

Tropomyosin from porcine muscle has been used:

  • as a positive control in actin- α-tropomyosin (TPM1) co-sedimentation analysis and assay
  • in analyzing the anti-adhesive properties of fish tropomyosins
  • in far Western blot analysis

Actions biochimiques/physiologiques

Tropomyosin is associated with the actin cytoskeleton and plays a role in stabilizing and regulating the interactions of actin with other actin-binding proteins. It also plays a role in muscle contraction by regulating the interactions of actin with myosin.
Tropomyosin together with troponin, regulates the binding of myosin to actin. Tropomyosin is a dimeric coiled-coil protein forming continuous polymers along the myosin-binding groove of actin. Various tropomyosin isoforms help confer structure and function to actins in the cytoskeleton and striated muscle function. Dimeric protein that assumes an α-helical coiled-coil conformation. In the absence of Ca2+, it inhibits muscle contractility by blocking the myosin binding sites on actin.

Remarque sur l'analyse

SDS electrophoresis shows two major bands.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)


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Consulter la Bibliothèque de documents

A novel alpha-tropomyosin mutation associates with dilated and non-compaction cardiomyopathy and diminishes actin binding
Judith B A, et al.
Biochimica et Biophysica Acta - Molecular Cell Research, 1833(4), 833-839 (2013)
Vertebrate tropomyosin: distribution, properties and function
Perry S V, et al.
Journal of Muscle Research and Cell Motility, 5?49-5?49 (2001)
A systematic nomenclature for mammalian tropomyosin isoforms
Michael A G, et al.
Journal of Muscle Research and Cell Motility, 147?153-147?153 (2015)
The molecular basis for tropomyosin isoform diversity
James P L M, et al.
Bioessays (1991)
M I Hamilton et al.
Clinical and experimental immunology, 99(3), 404-411 (1995-03-01)
Ulcerative colitis (UC) has a proposed autoimmune pathogenesis. A 40-kD antigen (P40) has been isolated from UC colon, bound to immunoglobulin. Tropomyosin has been reported as the target antigen of a MoAb (7E12H12) raised against P40. We set out to

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