T2400
Tropomyosin from porcine muscle
lyophilized powder (contains Tris buffer salts)
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About This Item
Produits recommandés
Source biologique
Porcine muscle
Niveau de qualité
Pureté
≥70% protein basis (biuret)
Forme
lyophilized powder (contains Tris buffer salts)
Technique(s)
electrophoresis: suitable
Température de stockage
−20°C
Description générale
Tropomyosin is a member of the actin filament binding protein family. It is a ubiquitous protein present in animals and fungi. Tropomyosin is made up of two α-helical chains arranged as a coiled-coil. It exists over twenty isoforms, in different eukaryotic cells like skeletal, cardiac, and smooth muscles, brain, platelets, fibroblasts, platelets, and other non-muscle cells.
Application
Tropomyosin from porcine muscle has been used:
- as a positive control in actin- α-tropomyosin (TPM1) co-sedimentation analysis and assay
- in analyzing the anti-adhesive properties of fish tropomyosins
- in far Western blot analysis
Actions biochimiques/physiologiques
Tropomyosin is associated with the actin cytoskeleton and plays a role in stabilizing and regulating the interactions of actin with other actin-binding proteins. It also plays a role in muscle contraction by regulating the interactions of actin with myosin.
Tropomyosin together with troponin, regulates the binding of myosin to actin. Tropomyosin is a dimeric coiled-coil protein forming continuous polymers along the myosin-binding groove of actin. Various tropomyosin isoforms help confer structure and function to actins in the cytoskeleton and striated muscle function. Dimeric protein that assumes an α-helical coiled-coil conformation. In the absence of Ca2+, it inhibits muscle contractility by blocking the myosin binding sites on actin.
Remarque sur l'analyse
SDS electrophoresis shows two major bands.
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
Certificats d'analyse (COA)
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A novel alpha-tropomyosin mutation associates with dilated and non-compaction cardiomyopathy and diminishes actin binding
Judith B A, et al.
Biochimica et Biophysica Acta - Molecular Cell Research, 1833(4), 833-839 (2013)
Vertebrate tropomyosin: distribution, properties and function
Perry S V, et al.
Journal of Muscle Research and Cell Motility, 5?49-5?49 (2001)
A systematic nomenclature for mammalian tropomyosin isoforms
Michael A G, et al.
Journal of Muscle Research and Cell Motility, 147?153-147?153 (2015)
The molecular basis for tropomyosin isoform diversity
James P L M, et al.
Bioessays (1991)
M I Hamilton et al.
Clinical and experimental immunology, 99(3), 404-411 (1995-03-01)
Ulcerative colitis (UC) has a proposed autoimmune pathogenesis. A 40-kD antigen (P40) has been isolated from UC colon, bound to immunoglobulin. Tropomyosin has been reported as the target antigen of a MoAb (7E12H12) raised against P40. We set out to
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