T2036

apo-Transferrine human

powder, BioReagent, suitable for cell culture, ≥98% (agarose gel electrophoresis)

• Synonyme(s) : Sidérophiline, Transferrine humaine
• Numéro CAS: 11096-37-0
• UNSPSC Code: 12352202
• EC Number: 234-318-8
• NACRES: NA.75
• MDL number: MFCD00130536
• eCl@ss: 34058011

Propriétés

• product line: BioReagent
• Quality Segment: 200
• assay: ≥98% (agarose gel electrophoresis)
• form: powder
• mol wt: 76-81 kDa
• concentration: ~25 mM
• technique(s): cell culture | mammalian: suitable
• impurities: HIV and HBsAg, source material tested negative, endotoxin, tested
• solubility: H₂O: 50 mg/mL
• cation traces: Fe: ≤0.005%
• UniProt accession no.: P02787
• shipped in: ambient
• storage temp.: −20°C
• Gene Information: human ... TF(7018)

Description

General description

Transferrin, majorly synthesized in the liver, is an abundant glycoprotein in the serum. The members of the transferrin superfamily have similar polypeptide folding patterns. Transferrin contains N- and C-terminal iron-binding homologous domains. Each of these domains is split into two subdomains having binding sites for iron and anions within the inter-subdomain cleft. The binding cleft opens and closes with iron releasing and iron binding. apo-Transferrin is an iron-free protein that arises after transferrin dissociates from its receptor. This product can be supplemented with iron or used to bind free iron present in media.

Application

apo-Transferrin human has been used:

• to culture human primary pancreatic endothelial cells, Het1As (non-tumorous esophagus cells), and immortalized human colonic epithelial cells (HCEC-1CT)
• to culture α mouse liver 12 (AML-12) (mature hepatocytes) cells
• as a source for human apo-transferrin for purification before crystallography

Biochem/physiol Actions

Transferrin is responsible for transporting iron from the sites of absorption and storage to the tissue cells. It maintains the levels of iron in biological fluids. The levels of transferrin may indicate the total iron-binding capacity (TIBC). Transferrin supplies the required iron for incorporation into hemoglobin within RBCs in the bone marrow. Iron deficiency causes an increase in the levels of transferrin. Pregnancy and estrogen administration can also raise the levels of transferrin. Chronic liver disease, renal insufficiency, malnutrition, and protein-losing enteropathies reduce the synthesis of transferrin.

Other Notes

Protéine de transport du fer du type non-hème.

Disclaimer

Ce produit, destiné à la recherche scientifique, est soumis à une réglementation spécifique en France, y compris pour les activités d′importation et d′exportation (Article L 1211-1 alinéa 2 du Code de la Santé Publique). L′acheteur (c′est-à-dire l′utilisateur FINAL) est tenu d′obtenir une autorisation d′importation auprès du ministère français de la recherche, mentionné à l′article L1245-5-1 II du Code de la Santé Publique. En commandant ce produit, vous confirmez détenir l′autorisation d′importation requise.

Informations sur la sécurité

• Classe de stockage: 11 - Combustible Solids
• wgk: WGK 3