Name: MISSION® siRNA Product Offerings
Brand: SIGMA

Custom DNA and RNA Oligos & qPCR Probes Product Resources

Custom Oligos & qPCR Probes Online Tools

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<h2>Predesigned siRNA</h2>
Extensive collection of human, mouse and rat siRNA. Multiple sequences are available for every gene, with rankings to indicate performance.
<ul>
<li>Scale:&nbsp;10, 25, 50, and 250 nmol</li>
<li>Purification:&nbsp;Desalt or HPLC</li>
<li>Sequence Length:&nbsp;21 bases</li>
<li>Modifications:&nbsp;Not available for this item</li>
<li>Quality Control:&nbsp;Individual strands are analyzed by mass spectrometry. Depending on manufacturing site, PAGE may be used to assess siRNA duplexes. siRNA quantity is systematically validated by UV absorbance at 260 nm.</li>
<li>Format:&nbsp;Supplied dry</li>
</ul>

Positive Control siRNA.
<ul>
<li>Validated human and rat positive controls to simplify transfection optimization.</li>
</ul>

<h2>siRNA Controls</h2>
Universal Negative Controls
<ul>
<li>Two distinct siRNA control sequences designed for use in Human, Mouse, or Rat cells.</li>
<li>Validated with Agilent 40K human gene arrays to ensure no significant nonspecific gene interactions.</li>
<li>Functionally validated to show no immune response via qRT PCR.</li>
</ul>

<h2>Custom miRNA</h2>
Custom miRNA sequences with a wide range of modifications, purifications, and quantities to tailor your unique sequence.
<ul>
<li>Amount:&nbsp;2 OD (10 nmol), 5 OD (25 nmol), 10 OD (50 nmol) &amp; 50 OD (250 nmol)</li>
<li>Purification: Desalt or HPLC</li>
<li>Length: 19-27mer duplexes</li>
<li>Modifications: Biotin, Phosphate, Amino-Modifier C7, 6-FAM, Cyanine 3, Cyanine 5, Cyanine 5.5, 2’-OMe, Phosphorothioate, Thiol-Modifier C6 S-S and Thiol-Modifier C3</li>
<li>Quality Control: Individual strands are analyzed by mass spectrometry.&nbsp; Depending on the manufacturing site, PAGE may be used to assess miRNA duplexes.&nbsp; miRNA quantity is systematically validated by UV absorbance at 260 nm.</li>
<li>For additional modifications and purification requests, email <a href="mailto:sirnarequest@sial.com">sirnarequest@sial.com</a></li>
</ul>

<h2>MISSION®&nbsp;siRNA Transfection Reagent</h2>
<ul>
<li>Superior gene silencing in a wide variety of eukaryotic cell types</li>
<li>For use with siRNA, esiRNA, miRNA mimics and Synthetic miRNA inhibitors</li>
</ul>

<h2>Fast siRNA</h2>
Expedite your siRNA order! Orders are prioritized in the production process with a turn-around-time of only 3 days.
<ul>
<li>FAST siRNA is delivered in solution at concentrations of 50 µM (2 OD) or 100 µM (5 OD).</li>
<li>US orders must be received by 11 AM CST. European orders must be received by 11 AM CET.</li>
<li>Easy to use Fast siRNA ordering tool.</li>
</ul>

<h2>Custom siRNA</h2>
Custom siRNA sequences with a wide range of modifications, purifications and quantities to tailor your unique sequence.
<ul>
<li>Scale:&nbsp;10, 25, 50, and 250 nmol</li>
<li>Purification:&nbsp;Desalt or HPLC</li>
<li>Sequence Lengths:&nbsp;19 - 27 bases</li>
<li>Modifications:&nbsp;Comprehensive offering of modifications and dyes (2'-O-Methyl, Amine, Cyanine dyes, 6-FAM, Biotin, Phosphate, Thiol, Abasic, Phosphorothioate linkages, 2'-Fluoro).</li>
<li>Quality Control:&nbsp;Individual strands are analyzed by mass spectrometry. Depending on manufacturing site, PAGE may be used to assess siRNA duplexes. siRNA quantity is systematically validated by UV absorbance at 260 nm.</li>
<li>For additional purifications, sequence length or modifications, 
email&nbsp;<a href="mailto:sirnarequest@sial.com">sirnarequest@sial.com</a></li>
<li>Custom siRNA sequence design available for a variety of species, 
<a href="/services/support/sirna-design-service" target="_self">Learn more</a></li>
</ul>

<h2>iScale Oligos™ - siRNA</h2>
siRNA provided in larger quantities for&nbsp;in vivo, high throughput or diagnostic projects.
<ul>
<li>Amount:&nbsp;1 – 10 mg, 50 mg – 1 g</li>
<li>Purification:&nbsp;Desalt, RPC (cartridge purified), HPLC and&nbsp;In vivo quality</li>
<li>Sequence Length:&nbsp;19 - 45 bases</li>
<li>Comprehensive offering of modifications including dyes, amines, biotin, linkers, etc.</li>
<li>Quality Control:&nbsp;Individual strands are analyzed by mass spectrometry. Depending on manufacturing site, PAGE may be used to assess siRNA duplexes. siRNA quantity is systematically validated by UV absorbance at 260 nm.</li>
<li>In vivo&nbsp;option includes HPLC-purification to &gt;/= 90%, desalt, filtration and endotoxin testing.</li>
<li>To inquire for availability of additional quantities, modifications or purifications, email&nbsp;<a href="mailto:sirnarequest@sial.com">sirnarequest@sial.com</a></li>
</ul>

<h2>RNA Oligos</h2>
<ul>
<li>Scale: 0.05, 0.2, 1.0 µmol</li>
<li>Purification: Desalt, HPLC and PAGE</li>
<li>Sequence Length: 7 - 45 bases</li>
<li>Modification options available</li>
<li>Quality Control: 100% Mass Spectrometry</li>
<li>Format: Supplied dry</li>
<li>Learn more about&nbsp;<a href="/technical-documents/technical-article/genomics/pcr/custom-rna-oligos" target="_self">RNA Oligos</a></li>
</ul>

<h2>PREDESIGNED&nbsp;&amp; Custom esiRNA</h2>
Biologically-prepared, esiRNA are comprised of a heterogeneous pool of siRNA (natural RNA, no modifications) that all target the same mRNA sequence.
<ul>
<li>Format:&nbsp;Individual esiRNA, panels, and libraries available. Individual: 20 or 50 µg lyophilized in tubes. Panels &amp; libraries: 1, 2.5, or 5 µg in solution in plates</li>
<li>Purification:&nbsp;Q-Sepharose separation, isopropanol precipitation &amp; ethanol washing</li>
<li>Sequence Form:&nbsp;Pool of hundreds of siRNA with an average duplex length of 21 bp</li>
<li>Quality Control:&nbsp;PCR product of cDNA clone is analyzed by gel electrophoresis &amp; DNA sequencing; digestion reaction is analyzed by gel electrophoresis</li>
<li><a href="/technical-documents/technical-article/genomics/gene-expression-and-silencing/mission-esirna-specifications">Learn more</a></li>
</ul>

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MISSION® siRNA Product Offerings Custom and Predesigned siRNA; Synonyms: Custom siRNA,High-throughput siRNA,Predesigned siRNA,siRNA,siRNA pools at Sigma-Aldrich

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MISSION^® siRNA Product Offerings

Custom and Predesigned siRNA

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